| Immunoaffinity purification and characterization of RNA polymerase from Shewanella oneidensis. | |
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MedLine Citation:
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PMID: 17507238 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Shewanella oneidensis is of particular interest for research because of its unique ability to use a variety of metals as final respiratory electron acceptors and reduce them into insoluble oxides. A collection of monoclonal antibodies (mAbs) that were prepared towards Escherichia coli RNA polymerase (RNAP) was tested for reactivity with proteins extracted from S. oneidensis. Two polyol-responsive monoclonal antibodies (PR-mAbs) were used to purify RNA polymerase from S. oneidensis using immunoaffinity purification techniques. A collection of mAbs towards E. coli sigma subunits was also examined for cross-reactivity with S. oneidensis proteins. Reactions were identified with mAbs to E. coli sigma(70) and sigma(54). These mAbs will be useful tools for immunoaffinity purifying and studying the transcriptional machinery of S. oneidensis. |
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Authors:
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Mitchell D Probasco; Nancy E Thompson; Richard R Burgess |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-04-10 |
Journal Detail:
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Title: Protein expression and purification Volume: 55 ISSN: 1046-5928 ISO Abbreviation: Protein Expr. Purif. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-08-22 Completed Date: 2007-11-01 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 9101496 Medline TA: Protein Expr Purif Country: United States |
Other Details:
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Languages: eng Pagination: 23-30 Citation Subset: IM |
Affiliation:
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McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal
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immunology Antibody Specificity Bacterial Proteins / chemistry, isolation & purification* Blotting, Western Chromatography, Affinity DNA-Directed RNA Polymerases / chemistry, isolation & purification* Shewanella / enzymology* |
| Grant Support | |
ID/Acronym/Agency:
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GM-28575/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Bacterial Proteins; EC 2.7.7.6/DNA-Directed RNA Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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