Document Detail


Immunoaffinity purification and characterization of RNA polymerase from Shewanella oneidensis.
MedLine Citation:
PMID:  17507238     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Shewanella oneidensis is of particular interest for research because of its unique ability to use a variety of metals as final respiratory electron acceptors and reduce them into insoluble oxides. A collection of monoclonal antibodies (mAbs) that were prepared towards Escherichia coli RNA polymerase (RNAP) was tested for reactivity with proteins extracted from S. oneidensis. Two polyol-responsive monoclonal antibodies (PR-mAbs) were used to purify RNA polymerase from S. oneidensis using immunoaffinity purification techniques. A collection of mAbs towards E. coli sigma subunits was also examined for cross-reactivity with S. oneidensis proteins. Reactions were identified with mAbs to E. coli sigma(70) and sigma(54). These mAbs will be useful tools for immunoaffinity purifying and studying the transcriptional machinery of S. oneidensis.
Authors:
Mitchell D Probasco; Nancy E Thompson; Richard R Burgess
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-04-10
Journal Detail:
Title:  Protein expression and purification     Volume:  55     ISSN:  1046-5928     ISO Abbreviation:  Protein Expr. Purif.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-22     Completed Date:  2007-11-01     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  9101496     Medline TA:  Protein Expr Purif     Country:  United States    
Other Details:
Languages:  eng     Pagination:  23-30     Citation Subset:  IM    
Affiliation:
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology
Antibody Specificity
Bacterial Proteins / chemistry,  isolation & purification*
Blotting, Western
Chromatography, Affinity
DNA-Directed RNA Polymerases / chemistry,  isolation & purification*
Shewanella / enzymology*
Grant Support
ID/Acronym/Agency:
GM-28575/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Bacterial Proteins; EC 2.7.7.6/DNA-Directed RNA Polymerases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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