Document Detail


Immunoaffinity, capillary electrophoresis, and statistics for studying intact alpha 1-Acid glycoprotein isoforms as an atherothrombosis biomarker.
MedLine Citation:
PMID:  22976104     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Variations in the amino acid sequence, glycosylation, and/or other posttranslational modifications in glycoproteins give rise to different molecules of the glycoprotein called forms. Qualitative and/or quantitative alterations in these forms are related to pathophysiological situations in the individuals. In this study, a methodology to analyze these differences in forms of the alpha 1-acid glycoprotein (AGP) between healthy individuals and patients with two different vascular diseases is detailed. The whole methodology includes a sample preparation method based on immunochromatography, a capillary electrophoresis method for separation of AGP peaks (isoforms), and statistical methods (Linear Discriminant Analysis) for sample classification. As a result, it is shown that the methodology proposed allows studying the role of AGP isoforms as potential vascular disease biomarkers.
Authors:
Angel Puerta; Pedro J Martin-Alvarez; Sara Ongay; Jose C Diez-Masa; Mercedes de Frutos
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  919     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-09-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  215-30     Citation Subset:  IM    
Affiliation:
Institute of Organic Chemistry (IQOG-CSIC), Madrid, Spain.
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