| Immunoaffinity, capillary electrophoresis, and statistics for studying intact alpha 1-Acid glycoprotein isoforms as an atherothrombosis biomarker. | |
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MedLine Citation:
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PMID: 22976104 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Variations in the amino acid sequence, glycosylation, and/or other posttranslational modifications in glycoproteins give rise to different molecules of the glycoprotein called forms. Qualitative and/or quantitative alterations in these forms are related to pathophysiological situations in the individuals. In this study, a methodology to analyze these differences in forms of the alpha 1-acid glycoprotein (AGP) between healthy individuals and patients with two different vascular diseases is detailed. The whole methodology includes a sample preparation method based on immunochromatography, a capillary electrophoresis method for separation of AGP peaks (isoforms), and statistical methods (Linear Discriminant Analysis) for sample classification. As a result, it is shown that the methodology proposed allows studying the role of AGP isoforms as potential vascular disease biomarkers. |
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Authors:
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Angel Puerta; Pedro J Martin-Alvarez; Sara Ongay; Jose C Diez-Masa; Mercedes de Frutos |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 919 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-09-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 215-30 Citation Subset: IM |
Affiliation:
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Institute of Organic Chemistry (IQOG-CSIC), Madrid, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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