| Immuno-spin trapping: detection of protein-centered radicals. | |
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MedLine Citation:
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PMID: 23045116 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Protein-centered radicals are involved in biological oxidative damage induced by drugs, environmental hazards, and cellular reactive oxygen species. Presently, the technique most widely used to study protein-centered radicals is electron spin resonance (ESR; also known as electron paramagnetic resonance, EPR); used either directly or in combination with the spin-trapping technique. Protein-centered radicals may be trapped with the nitrone spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) forming DMPO-radical adducts. However, after a few minutes these adducts decay, often by oxidation to DMPO-protein radical-derived nitrone adducts, which are ESR-silent species. Because nitrone adducts are not free radicals and their formation involves the creation of a covalent linkage, they are stable long after the ESR signal decays. In the new alternative technique of immuno-spin trapping, nitrone adducts are detected by using an antibody, i.e., anti-DMPO, that recognizes their nitrone moiety. Immuno-spin trapping is a simple, reliable, affordable, sensitive, and specific approach to detecting protein-centered radicals, and its development brings the power of immunoassays to bear on the field of toxicology of free radical-mediated biological damage. |
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Authors:
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Dario C Ramirez; Ronald P Mason |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.] Volume: Chapter 17 ISSN: 1934-9262 ISO Abbreviation: Curr Protoc Toxicol Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2012-10-09 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9816330 Medline TA: Curr Protoc Toxicol Country: United States |
Other Details:
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Languages: eng Pagination: Unit17.7 Citation Subset: IM |
Affiliation:
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National Institute of Environmental Health Science, National Institutes of Health, Research Triangle Park, North Carolina. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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