Document Detail


Immuno-isolation and culture of biliary epithelial cells from normal human liver.
MedLine Citation:
PMID:  2691499     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biliary epithelial cells (BEC) lining the intra-hepatic biliary ducts are the site of damage in several immunologically mediated liver diseases. BEC are difficult to isolate since they represent only 5% of the total cell number in normal liver. In this communication, a novel method for their isolation from normal liver is presented using a monoclonal antibody (HEA125) with specificity for an epithelial cell surface glyco-protein reported to be expressed in liver only by biliary epithelium. By combining differential density centrifugation and immuno-magnetic separation using HEA125 pure BEC (10(5) cells/g fresh tissue) were prepared routinely. These cells were maintained in culture for up to 4 weeks with significant increases in cell numbers. The ability to prepare BEC from human liver offers an opportunity to develop In Vitro models to investigate the aetiology of diseases of intra-hepatic biliary epithelium.
Authors:
R Joplin; A J Strain; J M Neuberger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  25     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-02-22     Completed Date:  1990-02-22     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1189-92     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology*
Antibody Specificity
Bile Ducts, Intrahepatic / cytology*,  immunology
Cell Separation / methods
Cells, Cultured
Epithelial Cells
Humans
Immunoenzyme Techniques
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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