Document Detail


Immunization with structural and non-structural proteins of Theiler's murine encephalomyelitis virus alters demyelinating disease.
MedLine Citation:
PMID:  22403027     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Theiler's murine encephalomyelitis virus (TMEV) causes a demyelinating disease similar to multiple sclerosis in the central nervous system (CNS) of susceptible SJL/J mice. Immune responses to TMEV contribute to viral clearance as well as to demyelination. We constructed recombinant vaccinia viruses (VV) that encode each or all of the capsid proteins (VV(VP1), VV(VP2), VV(VP3), VV(VP4), and VV(all)) or non-structural proteins (VV(P2), VV(P2P3), and VV(3'P3)) of the Daniels strain of TMEV. To determine the role of each of the coding regions of TMEV in vivo, we immunized SJL/J mice with each recombinant VV, with or without subsequent TMEV infection. The groups of mice were compared clinically, immunologically, and histologically. No mice immunized with any recombinant VV without subsequent TMEV infection developed demyelination. However, antibody responses to TMEV were detected in mice immunized with VV(all). In addition, in some mice, VV(P2) immunization induced mild meningitis. VV(VP3) or VV(VP4) immunization of mice prior to TMEV infection ameliorated TMEV-induced pathology or clinical signs of disease. The beneficial effect of VP4 immunization was also seen through DNA immunization with a plasmid encoding VP4 and leader prior to TMEV infection. Therefore, vaccination against not only surface capsid proteins (VV(VP3) and VV(all)) but also non-surface capsid protein (VV(VP4)), and non-structural proteins (VV(P2)) can elicit immune responses to virus or modulate subsequent viral-induced CNS disease.
Authors:
Ikuo Tsunoda; Jane E Libbey; Robert S Fujinami
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-09
Journal Detail:
Title:  Journal of neurovirology     Volume:  18     ISSN:  1538-2443     ISO Abbreviation:  J. Neurovirol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-24     Completed Date:  2013-06-19     Revised Date:  2013-12-10    
Medline Journal Info:
Nlm Unique ID:  9508123     Medline TA:  J Neurovirol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  127-37     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Capsid Proteins / genetics,  immunology*
Central Nervous System / immunology*,  pathology,  virology
Demyelinating Diseases / immunology,  pathology,  prevention & control*,  virology
Female
Genetic Engineering
Immunization
Meningitis, Viral / etiology,  virology
Mice
Open Reading Frames
Recombinant Proteins / genetics,  immunology
Theilovirus / genetics*,  immunology
Vaccinia virus / genetics,  immunology
Viral Nonstructural Proteins / genetics,  immunology*
Viral Vaccines / administration & dosage*,  adverse effects,  therapeutic use
Grant Support
ID/Acronym/Agency:
NS34497/NS/NINDS NIH HHS; P20 RR018724/RR/NCRR NIH HHS; P20 RR018724-07/RR/NCRR NIH HHS; P20-RR018724/RR/NCRR NIH HHS; R01 NS034497-08/NS/NINDS NIH HHS; R21 NS059724-01A2/NS/NINDS NIH HHS; R21NS059724/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Capsid Proteins; 0/Recombinant Proteins; 0/Viral Nonstructural Proteins; 0/Viral Vaccines
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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