| Immunization with Toscana virus N-Gc proteins protects mice against virus challenge. | |
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MedLine Citation:
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PMID: 18355889 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Toscana virus (TOSV) is an emerging virus, circulating in the Mediterranean area, that is responsible for aseptic meningitis, meningoencephalitis, and encephalitis. The development of a vaccine that could provide complete protection from TOSV infection is needed. In this study we investigated the capacity of TOSV structural proteins, nucleocapsid protein N and the two Gc and Gn glycoproteins, produced as recombinant proteins, in an animal model. In particular, we investigated their role in inducing specific and protective immune responses against virus infection. Mice were immunized intraperitoneally using TOSV antigens singly or in combination. The results show that only the N-Gc combination was able to protect 100% of animals from a lethal challenge with a neurovirulent strain of TOSV. This potential vaccine induces high serum antibody titres with neutralizing activity and it is safe for animals. Moreover, immunization induces a virus specific cell-mediated immune response, in particular a CD8+ T cell response associated with a marked expression of interferon gamma. These results indicate that the N+Gc viral antigen combination could be useful for future development of a vaccine controlling the spread of this emerging virus that could pose a new threat for humans. |
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Authors:
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Gianni Gori Savellini; Giuseppa Di Genova; Chiara Terrosi; Paola Di Bonito; Colomba Giorgi; Melissa Valentini; Jean-Denis Docquier; Maria Grazia Cusi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-19 |
Journal Detail:
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Title: Virology Volume: 375 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-19 Completed Date: 2008-07-15 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 521-8 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, Section of Microbiology, University of Siena, Siena, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Viral / blood CD8-Positive T-Lymphocytes / immunology, metabolism Cells, Cultured Cytotoxicity Tests, Immunologic Female Humans Immunization Immunization Schedule Injections, Intraperitoneal Interferon-gamma / biosynthesis Mice Mice, Inbred BALB C Neutralization Tests Nucleocapsid Proteins / immunology* Phlebotomus Fever / immunology*, prevention & control* Sandfly fever Naples virus / immunology* Spleen / immunology Vaccines, Synthetic / administration & dosage Viral Envelope Proteins / immunology* Viral Vaccines / administration & dosage |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/Nucleocapsid Proteins; 0/Vaccines, Synthetic; 0/Viral Envelope Proteins; 0/Viral Vaccines; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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