Document Detail


Immunization of normal human splenocytes in vitro to produce human monoclonal antibodies to cellular antigens.
MedLine Citation:
PMID:  3672490     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have obtained human monoclonal antibodies to polymorphic cell surface determinants by immunizing normal human splenecytomy in vitro to allogeneic cells. Splenocytes from young patients undergoing splenectomy secondary to traumatic injury are separated into T and B lymphocyte populations. The T lymphocytes are irradiated with 1500 rad to selectively inactivate T suppressors. Responder T and B cells are then recombined at a 1:1 ratio. Maximal IgM and IgG production is obtained when pokeweed mitogen and irradiated stimulator cells are added to the cultures. Stimulator cell specific antibody levels peak at day 7 of in vitro immunization, and fall thereafter. Fusion of the immunized splenocytes to a human fusion partner as early as day 4 results in hybridomas secreting antibodies to cellular antigens. Transformation by EBV, expansion and fusion of the immunized cell line also yield hybridomas secreting stimulator cell specific antibody.
Authors:
C M Hulette; R B Effros; R L Walford
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Tissue antigens     Volume:  30     ISSN:  0001-2815     ISO Abbreviation:  Tissue Antigens     Publication Date:  1987 Jul 
Date Detail:
Created Date:  1987-12-17     Completed Date:  1987-12-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0331072     Medline TA:  Tissue Antigens     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  25-33     Citation Subset:  IM    
Affiliation:
UCLA School of Medicine, Department of Pathology.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology*
Cell Fusion
Cells, Cultured
Dose-Response Relationship, Immunologic
HLA Antigens / immunology
Humans
Hybridomas / immunology*
Immunization
Mitogens
Spleen / cytology,  immunology*
Time Factors
Grant Support
ID/Acronym/Agency:
AG 05309/AG/NIA NIH HHS; AI 10088/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/HLA Antigens; 0/Mitogens

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