| Immunization of burn-patients with a Pseudomonas aeruginosa outer membrane protein vaccine elicits antibodies with protective efficacy. | |
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MedLine Citation:
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PMID: 10699346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to determine whether the antibodies raised in burn patients by active immunization with a Pseudomonas aeruginosa OMPs vaccine have a protective efficacy against infection with P. aeruginosa. The binding patterns with P. aeruginosa OMPs of immunized burn patient sera were similar to the sera of immunized healthy humans as determined by immunoblot and immunoprecipitation analyses. The sera pooled from immunized burn patients after three immunizations showed a significantly higher opsonophagocytic-killing activity than the corresponding pre-immune sera, while the sera from unimmunized patients collected at the same day did not. Passive immunization of mice with post-immune sera of burn patients significantly enhanced the survival rate upon a lethal challenge with P. aeruginosa compared to the pre-immune sera, indicating the protective ability of the antibodies induced in burn patients by immunization. These results suggest that anti-P. aeruginosa OMPs antibodies elicited in burn patients by active immunization are protective against infection with P. aeruginosa, and provide a rational for further development of the vaccine for prevention against P. aeruginosa infection in burn patients. |
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Authors:
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N G Lee; S B Jung; B Y Ahn; Y H Kim; J J Kim; D K Kim; I S Kim; S M Yoon; S W Nam; H S Kim; W J Park |
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Publication Detail:
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Type: Clinical Trial; Clinical Trial, Phase II; Journal Article; Randomized Controlled Trial |
Journal Detail:
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Title: Vaccine Volume: 18 ISSN: 0264-410X ISO Abbreviation: Vaccine Publication Date: 2000 Mar |
Date Detail:
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Created Date: 2000-05-09 Completed Date: 2000-05-09 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1952-61 Citation Subset: IM |
Affiliation:
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R&D Center of Bioscience, Institute of Science and Technology, Cheiljedang Corp., Ichon, Kyonggi, South Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Bacterial / biosynthesis*, blood, therapeutic use Antigen-Antibody Reactions Bacterial Outer Membrane Proteins / immunology, metabolism, therapeutic use* Bacterial Vaccines / immunology, therapeutic use* Burns / immunology*, microbiology* Humans Immunization, Passive / methods Immunoglobulin G / biosynthesis, blood, immunology Male Mice Mice, Inbred ICR Opsonin Proteins / immunology Phagocytosis / immunology Pseudomonas Infections / prevention & control* Pseudomonas aeruginosa / immunology* Rabbits Vaccines, Inactivated / immunology, therapeutic use |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Vaccines; 0/Immunoglobulin G; 0/Opsonin Proteins; 0/Vaccines, Inactivated |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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