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Immunisation of hamsters against Clostridium difficile infection using the Cwp84 protease as antigen.
MedLine Citation:
PMID:  21707776     Owner:  NLM     Status:  Publisher    
Clostridium difficile is a pathogen responsible for diarrhoea and colitis, particularly after antibiotic treatment. We evaluated the C. difficile protease Cwp84, found to be associated with the S-layer proteins, as a vaccine antigen to limit the C. difficile intestinal colonisation and therefore the development of the infection in a clindamycin-treated hamster model. First, we evaluated the immune response and the animal death protection induced by several immunisation routes: rectal, intragastric and subcutaneous. Antibody production was variable according to the immunisation routes. In addition, serum Cwp84 antibody titres did not always correlate with animal protection after challenge with a toxigenic C. difficile strain. The best survival rate was observed with the rectal route of immunization. Then, in a second assay, we selected this immunisation route to perform a larger immunisation assay including a Cwp84 immunised group and a control group. C. difficile intestinal colonisation and survival rate, as well as the immune response were examined. C. difficile hamster challenge resulted in a 26% weaker and slower C. difficile intestinal colonisation by in the immunised group. Furthermore, the hamster survival in the Cwp84 immunised group was 33% greater than that of the control group with a significant statistical difference.
Séverine Péchiné; Cécile Denève; Alban Le Monnier; Sandra Hoys; Claire Janoir; Anne Collignon
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-24
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  -     ISSN:  1574-695X     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Université de Paris-Sud 11, Faculté de Pharmacie, EA 4043, USC INRA, 5 rue J.B. Clement, F-92296 Châtenay-Malabry cedex, France.
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