| Immune responses against chimeric DNA and protein vaccines composed of plpEN-OmpH and PlpEC-OmpH from Pasteurella multocida A:3 in mice. | |
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MedLine Citation:
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PMID: 23195557 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Pasteurella multocida is a pathogenic bacterium causing many diseases that are of significant economic importance to livestock industries. Outer membrane protein H (ompH) gene and two fragments of Pasteurella lipoprotein E (plpE) gene, namely plpEN and plpEC, were cloned from P. multocida A:3. Three DNA vaccine formulations, namely pCMV-ompH, pCMV-plpEN-ompH and pCMV-plpEC-ompH and two protein-based prototype vaccines, alum adjuvanted PlpEN-OmpH and PlpEC-OmpH, were generated. Antibody levels were induced in mice vaccinated with chimeric DNA or protein vaccines. A significant (p < 0.05) increase in serum IFN-g titer was obtained by vaccination with 100 μg of pCMV-ompH, pCMV-plpEC-ompH and PlpEC-OmpH. DNA vaccines did not provide protection upon intraperitoneal challenge with 10 LD50 of live P. multocida A:3. However, 40% protection was conferred by 100 μg of PlpEC-OmpH which was not statistically significant. These results showed that plpEN-ompH and plpEC-ompH chimeric DNA vaccines and alum adjuvanted PlpEN-OmpH or PlpEC-OmpH protein vaccines were immunogenic but not protective against P. multocida A:3 in mice. Prime-boost strategies, i.e. priming with DNA vaccines and boost with protein formulations or different adjuvants can be utilized to obtain significant protection. |
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Authors:
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Sezer Okay; Erkan Ozcengiz; Gülay Ozcengiz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta microbiologica et immunologica Hungarica Volume: 59 ISSN: 1217-8950 ISO Abbreviation: Acta Microbiol Immunol Hung Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-11-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9434021 Medline TA: Acta Microbiol Immunol Hung Country: Hungary |
Other Details:
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Languages: eng Pagination: 485-98 Citation Subset: IM |
Affiliation:
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Middle East Technical University Department of Biological Sciences 06800 Ankara Turkey. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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