Document Detail


Immune responses against chimeric DNA and protein vaccines composed of plpEN-OmpH and PlpEC-OmpH from Pasteurella multocida A:3 in mice.
MedLine Citation:
PMID:  23195557     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Pasteurella multocida is a pathogenic bacterium causing many diseases that are of significant economic importance to livestock industries. Outer membrane protein H (ompH) gene and two fragments of Pasteurella lipoprotein E (plpE) gene, namely plpEN and plpEC, were cloned from P. multocida A:3. Three DNA vaccine formulations, namely pCMV-ompH, pCMV-plpEN-ompH and pCMV-plpEC-ompH and two protein-based prototype vaccines, alum adjuvanted PlpEN-OmpH and PlpEC-OmpH, were generated. Antibody levels were induced in mice vaccinated with chimeric DNA or protein vaccines. A significant (p < 0.05) increase in serum IFN-g titer was obtained by vaccination with 100 μg of pCMV-ompH, pCMV-plpEC-ompH and PlpEC-OmpH. DNA vaccines did not provide protection upon intraperitoneal challenge with 10 LD50 of live P. multocida A:3. However, 40% protection was conferred by 100 μg of PlpEC-OmpH which was not statistically significant. These results showed that plpEN-ompH and plpEC-ompH chimeric DNA vaccines and alum adjuvanted PlpEN-OmpH or PlpEC-OmpH protein vaccines were immunogenic but not protective against P. multocida A:3 in mice. Prime-boost strategies, i.e. priming with DNA vaccines and boost with protein formulations or different adjuvants can be utilized to obtain significant protection.
Authors:
Sezer Okay; Erkan Ozcengiz; Gülay Ozcengiz
Related Documents :
24930717 - Evaluation of recombinant mycoplasma hyopneumoniae p97/p102 paralogs formulated with se...
21208907 - Phase i trial of tgf-{beta}2 antisense gm-csf gene-modified autologous tumor cell (tag)...
23175507 - Pre-existing immunity against vaccine vectors--friend or foe?
24513277 - Recombinant feline coronaviruses as vaccine candidates confer protection in spf but not...
23774257 - Retention of structure, antigenicity, and biological function of pneumococcal surface p...
6843257 - A new distending laryngoscope for diagnosis and microsurgery of the larynx.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta microbiologica et immunologica Hungarica     Volume:  59     ISSN:  1217-8950     ISO Abbreviation:  Acta Microbiol Immunol Hung     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9434021     Medline TA:  Acta Microbiol Immunol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  485-98     Citation Subset:  IM    
Affiliation:
Middle East Technical University Department of Biological Sciences 06800 Ankara Turkey.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Interference in quorum sensing signal transmission amongst microbial species.
Next Document:  Antiretroviral effect of 4-thio-uridylate against human immunodeficiency virus type 1.