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Immune response to n-terminal and c-terminal deletion mutants of Aspergillus fumigatus major allergen ASP F 3.
MedLine Citation:
PMID:  23105608     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The ubiquitous fungus Aspergillus fumigatus causes allergic rhinitis, asthma, sinusitis and allergic bronchopulmonary aspergillosis. A number of major allergens from A. fumigatus are purified, but their structure-function role in the pathogenesis of disease is not known. Such information is essential for devising alternative therapy of fungal allergic diseases. In the present study, N-terminal and C-terminal deletion mutants ofAsp f 3 were constructed and their immunopathological responses studied in a mice model of allergy. Three mutants viz,Asp f 3 (aa 33-168), (aa 1-142), and (aa 23-142) were made by deleting certain amino acids from epitopic regions of full lengthAsp f 3, a major allergen of A. furnigatus. TheAsp f 3 and three mutated proteins were expressed in pET vector. The C-terminal deletion mutantAsp f 3 (aa 1-142) induced elevated IFN-γ but low levels of IL-4 by spleen cells. This mutant also showed significant downregulation of peripheral blood eosinophils and lung inflammation in immunized mice. The N-terminal deletion mutantAsp f 3 (aa 33-168) also exhibited an immuno-suppressive effect in terms of IgE production and induction of Th2 cytokine. The results indicate thatrAsp f 3 and its deletion mutants induced distinct immune-inflammatory responses in mice on challenge with these proteins. The non-IgE binding deletion mutants ofAsp f 3 (aa 1-142 and aa 33-168) could deviate Th2 immune response with a concomitant reduction in airway inflammation and infiltration of inflammatory cells.
Bhanu P Singh; Banani Banerjee; Puspanita Naik; Jordan N Fink; Viswanath P Kurup
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Indian journal of clinical biochemistry : IJCB     Volume:  21     ISSN:  0970-1915     ISO Abbreviation:  Indian J Clin Biochem     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2012-10-29     Completed Date:  2012-10-30     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  8708303     Medline TA:  Indian J Clin Biochem     Country:  India    
Other Details:
Languages:  eng     Pagination:  20-7     Citation Subset:  -    
Allergy and Immunology Section, Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, 110007 Delhi, India.
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