| Immune response to n-terminal and c-terminal deletion mutants of Aspergillus fumigatus major allergen ASP F 3. | |
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MedLine Citation:
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PMID: 23105608 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The ubiquitous fungus Aspergillus fumigatus causes allergic rhinitis, asthma, sinusitis and allergic bronchopulmonary aspergillosis. A number of major allergens from A. fumigatus are purified, but their structure-function role in the pathogenesis of disease is not known. Such information is essential for devising alternative therapy of fungal allergic diseases. In the present study, N-terminal and C-terminal deletion mutants ofAsp f 3 were constructed and their immunopathological responses studied in a mice model of allergy. Three mutants viz,Asp f 3 (aa 33-168), (aa 1-142), and (aa 23-142) were made by deleting certain amino acids from epitopic regions of full lengthAsp f 3, a major allergen of A. furnigatus. TheAsp f 3 and three mutated proteins were expressed in pET vector. The C-terminal deletion mutantAsp f 3 (aa 1-142) induced elevated IFN-γ but low levels of IL-4 by spleen cells. This mutant also showed significant downregulation of peripheral blood eosinophils and lung inflammation in immunized mice. The N-terminal deletion mutantAsp f 3 (aa 33-168) also exhibited an immuno-suppressive effect in terms of IgE production and induction of Th2 cytokine. The results indicate thatrAsp f 3 and its deletion mutants induced distinct immune-inflammatory responses in mice on challenge with these proteins. The non-IgE binding deletion mutants ofAsp f 3 (aa 1-142 and aa 33-168) could deviate Th2 immune response with a concomitant reduction in airway inflammation and infiltration of inflammatory cells. |
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Authors:
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Bhanu P Singh; Banani Banerjee; Puspanita Naik; Jordan N Fink; Viswanath P Kurup |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Indian journal of clinical biochemistry : IJCB Volume: 21 ISSN: 0970-1915 ISO Abbreviation: Indian J Clin Biochem Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2012-10-29 Completed Date: 2012-10-30 Revised Date: 2013-03-21 |
Medline Journal Info:
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Nlm Unique ID: 8708303 Medline TA: Indian J Clin Biochem Country: India |
Other Details:
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Languages: eng Pagination: 20-7 Citation Subset: - |
Affiliation:
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Allergy and Immunology Section, Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, 110007 Delhi, India. |
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