| Immune response pattern of the popliteal lymph nodes of dogs with visceral leishmaniasis. | |
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MedLine Citation:
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PMID: 20499098 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study aimed to estimate the cell response and parasite load in the popliteal lymph nodes of dogs with visceral leishmaniasis (VL), comparing these findings with the clinical staging of the disease. From the necropsy, 33 dogs were classified as symptomatic (S), asymptomatic (A), or oligosymptomatic (O). Cytology and histopathology were used to determine any presence of microscopic lesions and immunohistochemistry, for parasite load. Dog hyperimmune serum was used as the primary antibody. The inflammatory infiltrate in lymph nodes consisted of macrophages and plasmocytes. The granulomas invaded the trabecular and sinusoid regions and sometimes compressed the lymphocytes of the cortical region (atrophy) and medullary cord cells. Parasite load intensity was unrelated to the density of the macrophages infiltrating the lymph node. Significant differences in parasite load (P < 0.05) were observed between the three groups of infected dogs. Follicular hyperplasia of the cortical region occurred among A and O, while follicular atrophy predominated among S. The parasite load was the greatest among S, followed by O. It can be concluded that, regardless of clinical condition, the most evident cell response consisted of macrophages and plasmocytes. Lymphoid atrophy was observed among animals with intense granulomatous reaction and high parasite load, such as among the symptomatic dogs (P < 0.05). Likewise, the oligosymptomatic dogs also presented high density of parasites in the lymph nodes. Thus, we can confirm that dogs with clinical manifestations of VL have an immune system that is less effective for controlling infection by Leishmania chagasi, thereby favoring parasite multiplication. |
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Authors:
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Pamela Rodrigues Reina Moreira; Lais Mendes Vieira; Mariana Macedo Costa de Andrade; Marcio de Barros Bandarra; Gisele Fabrino Machado; Danísio Prado Munari; Rosemeri de Oliveira Vasconcelos |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-25 |
Journal Detail:
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Title: Parasitology research Volume: 107 ISSN: 1432-1955 ISO Abbreviation: Parasitol. Res. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-02 Completed Date: 2010-11-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8703571 Medline TA: Parasitol Res Country: Germany |
Other Details:
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Languages: eng Pagination: 605-13 Citation Subset: IM |
Affiliation:
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Departamento de Patologia Animal, FCAV/UNESP, Jaboticabal, São Paulo, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Protozoan / blood Dog Diseases / immunology*, parasitology Dogs Female Immunity, Cellular Immunohistochemistry Leishmania infantum / immunology* Leishmaniasis, Visceral / immunology, parasitology, pathology, veterinary* Lymph Nodes / immunology*, parasitology, pathology Lymphatic Diseases / immunology, parasitology, pathology, veterinary* Male |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Protozoan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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