Document Detail


Immune response of chicken gut to natural colonization by gut microflora and to Salmonella enterica serovar enteritidis infection.
MedLine Citation:
PMID:  21555397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In commercial poultry production, there is a lack of natural flora providers since chickens are hatched in the clean environment of a hatchery. Events occurring soon after hatching are therefore of particular importance, and that is why we were interested in the development of the gut microbial community, the immune response to natural microbial colonization, and the response to Salmonella enterica serovar Enteritidis infection as a function of chicken age. The complexity of chicken gut microbiota gradually increased from day 1 to day 19 of life and consisted of Proteobacteria and Firmicutes. For the first 3 days of life, chicken cecum was protected by increased expression of chicken β-defensins (i.e., gallinacins 1, 2, 4, and 6), expression of which dropped from day 4 of life. On the other hand, a transient increase in interleukin-8 (IL-8) and IL-17 expression could be observed in chicken cecum on day 4 of life, indicating physiological inflammation and maturation of the gut immune system. In agreement, the response of chickens infected with S. Enteritidis on days 1, 4, and 16 of life shifted from Th1 (characterized mainly by induction of gamma interferon [IFN-γ] and inducible nitric oxide synthase [iNOS]), observed in younger chickens, to Th17, observed in 16-day-old chickens (characterized mainly by IL-17 induction). Active modification of chicken gut microbiota in the future may accelerate or potentiate the maturation of the gut immune system and increase its resistance to infection with different pathogens.
Authors:
Magdalena Crhanova; Helena Hradecka; Marcela Faldynova; Marta Matulova; Hana Havlickova; Frantisek Sisak; Ivan Rychlik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-05-09
Journal Detail:
Title:  Infection and immunity     Volume:  79     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-21     Completed Date:  2011-09-02     Revised Date:  2012-01-02    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2755-63     Citation Subset:  IM    
Affiliation:
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
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MeSH Terms
Descriptor/Qualifier:
Aging
Animals
Cecum / immunology,  microbiology
Chickens / immunology*,  microbiology*
Cytokines / metabolism
Enteritis
Enzyme-Linked Immunosorbent Assay
Gastrointestinal Tract / immunology*,  microbiology*
Immunity, Innate
Interleukin-17 / biosynthesis,  metabolism
Interleukin-8 / metabolism
Polymerase Chain Reaction
Poultry Diseases / immunology*,  microbiology
Proteobacteria
RNA, Ribosomal, 16S
Salmonella Infections, Animal / immunology*
Salmonella enteritidis / growth & development,  immunology*
Th1 Cells / immunology
Th17 Cells / immunology
beta-Defensins / metabolism
Chemical
Reg. No./Substance:
0/Cytokines; 0/Interleukin-17; 0/Interleukin-8; 0/RNA, Ribosomal, 16S; 0/beta-Defensins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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