| Immune requirements of post-exposure immunization with modified vaccinia Ankara of lethally infected mice. | |
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MedLine Citation:
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PMID: 20300179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Current prophylactic vaccines work via the induction of B and T cell mediated memory that effectively control further replication of the pathogen after entry. In the case of therapeutic or post-exposure vaccinations the situation is far more complex, because the pathogen has time to establish itself in the host, start producing immune-inhibitory molecules and spread into distant organs. So far it is unclear which immune parameters have to be activated in order to thwart an existing lethal infection. Using the mousepox model, we investigated the immunological mechanisms responsible for a successful post-exposure immunization with modified vaccinia Ankara (MVA). In contrast to intranasal application of MVA, we found that intravenous immunization fully protected mice infected with ectromelia virus (ECTV) when applied three days after infection. Intravenous MVA immunization induced strong innate and adaptive immune responses in lethally infected mice. By using various gene-targeted and transgenic mouse strains we show that NK cells, CD4 T cells, CD8 T cells and antibodies are essential for the clearance of ECTV after post-exposure immunization. Post-exposure immunization with MVA is an effective measure in a murine model of human smallpox. MVA activates innate and adaptive immune parameters and only a combination thereof is able to purge ECTV from its host. These data not only provide a basis for therapeutic vaccinations in the case of the deliberate release of pathogenic poxviruses but possibly also for the treatment of chronic infections and cancer. |
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Authors:
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Henning Lauterbach; Ronny Kassub; Juliane Pätzold; Jana Körner; Michael Brückel; Admar Verschoor; Paul Chaplin; Mark Suter; Hubertus Hochrein |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-11 |
Journal Detail:
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Title: PloS one Volume: 5 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2010 |
Date Detail:
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Created Date: 2010-03-19 Completed Date: 2011-01-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e9659 Citation Subset: IM |
Affiliation:
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Department of Research, Bavarian Nordic GmbH, Martinsried, Germany. henning.lauterbach@bavarian-nordic.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CD4-Positive T-Lymphocytes / cytology CD8-Positive T-Lymphocytes / cytology Communicable Disease Control Complement System Proteins Cytokines / metabolism Enzyme-Linked Immunosorbent Assay / methods Flow Cytometry / methods Immune System Immunization Killer Cells, Natural / cytology Mice Mice, Inbred C57BL Mice, Transgenic Vaccines / therapeutic use* Vaccinia virus / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Vaccines; 9007-36-7/Complement System Proteins |
| Comments/Corrections | |
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