| Immune profiling by multiple gene expression analysis in patients at-risk and with type 1 diabetes. | |
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MedLine Citation:
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PMID: 21414848 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There is a need for biomarkers to monitor the development and progression of type 1 DM. We analyzed mRNA expression levels for granzyme B, perforin, fas ligand, TNF-α, IFN-γ, Foxp3, IL-10, TGF-β, IL-4, IL-6, IL-17, Activation-induced cytidine deaminase (AID) and Immunoglobulin G gamma chain (IgG<gamma>) genes in peripheral blood of at-risk, new-onset and long-term type 1 DM , and healthy controls. The majority of the genes were suppressed in long-term type 1 DM compared to controls and new-onset patients. IFN-γ, IL-4 and IL-10 mRNA levels were significantly higher in new-onset compared to at-risk and long-term groups. There was decreased mRNA expression for AID and IgG<gamma> and up-regulation of IFN-γ with age in controls. Data suggest an overall depressed immunity in long-term type 1 DM. Increased gene expression levels for IFN-γ, IL-4 and IL-10 in new-onset patients from at-risk patients might be used as potential markers for progression of the disease. |
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Authors:
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Dongmei Han; Carlos A Leyva; Della Matheson; Davide Mineo; Shari Messinger; Bonnie B Blomberg; Ana Hernandez; Luigi F Meneghini; Gloria Allende; Jay S Skyler; Rodolfo Alejandro; Alberto Pugliese; Norma S Kenyon |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-02-24 |
Journal Detail:
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Title: Clinical immunology (Orlando, Fla.) Volume: 139 ISSN: 1521-7035 ISO Abbreviation: Clin. Immunol. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-17 Completed Date: 2011-08-08 Revised Date: 2012-09-20 |
Medline Journal Info:
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Nlm Unique ID: 100883537 Medline TA: Clin Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 290-301 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Diabetes Research Institute, Leonard Miller School of Medicine, University of Miami, FL 33136, USA. dhan@med.miami.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Biological Markers / blood Cytokines / biosynthesis, genetics*, immunology Diabetes Mellitus, Type 1 / blood, genetics*, immunology* Female Gene Expression Profiling / methods Humans Linear Models Male Multivariate Analysis RNA, Messenger / biosynthesis, genetics Reverse Transcriptase Polymerase Chain Reaction Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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U01 DK061037-01/DK/NIDDK NIH HHS; U01 DK061041/DK/NIDDK NIH HHS; U01 DK061041-01/DK/NIDDK NIH HHS; U01 DK070460-01/DK/NIDDK NIH HHS; U01DK061037/DK/NIDDK NIH HHS; UO1 DK070460/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Cytokines; 0/RNA, Messenger |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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