Document Detail


Immune modulation by probiotic strains: quantification of phagocytosis of Aeromonas salmonicida by leukocytes isolated from gut of rainbow trout (Oncorhynchus mykiss) using a radiolabelling assay.
MedLine Citation:
PMID:  17034857     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method is described for the rapid and sensitive assay of phagocytosis that utilizes radioactively labelled bacteria. With this method, we observed that phagocytosis of heat-inactivated Aeromonas salmonicida by leukocytes isolated from gut of rainbow trout fed with different viable probiotics (Lactococcus lactis subsp. lactis, Lactobacillus sakei, and Leuconostoc mesenteroides) was significantly higher (P<0.05) after 2 weeks of probiotic-feeding than the control group. However, only phagocytosis of live A. salmonicida by the leukocytes isolated from gut of rainbow trout fed with L. lactis subsp. lactis was significantly higher (P<0.05) than the control group.
Authors:
José Luis Balcázar; Daniel Vendrell; Ignacio de Blas; Imanol Ruiz-Zarzuela; Olivia Gironés; José Luis Muzquiz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-10
Journal Detail:
Title:  Comparative immunology, microbiology and infectious diseases     Volume:  29     ISSN:  0147-9571     ISO Abbreviation:  Comp. Immunol. Microbiol. Infect. Dis.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-06     Completed Date:  2007-01-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7808924     Medline TA:  Comp Immunol Microbiol Infect Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  335-43     Citation Subset:  IM    
Affiliation:
Laboratory of Fish Pathology, University of Zaragoza, Zaragoza 50013, Spain. balcazar@unizar.es
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MeSH Terms
Descriptor/Qualifier:
Aeromonas salmonicida / physiology
Animal Nutritional Physiological Phenomena
Animals
Lactobacillus / physiology
Lactococcus lactis / physiology
Leuconostoc / physiology
Leukocytes / immunology
Oncorhynchus mykiss / immunology*
Phagocytosis / physiology*
Probiotics / pharmacology*
Stomach / cytology
Tritium
Chemical
Reg. No./Substance:
10028-17-8/Tritium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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