Document Detail


Immune-mediated liver injury: prognostic value of CD4+, CD8+, and CD68+ in infants with extrahepatic biliary atresia.
MedLine Citation:
PMID:  16080928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Hepatic fibrosis and cirrhosis develop progressively in extrahepatic biliary atresia (EHBA) despite timely surgical intervention. PURPOSE: The aim of the study was to define CD4+ helper T lymphocytes, cytotoxic CD8+ T lymphocytes, and CD68+ (macrophages) infiltration of portal tracts and lobules and hepatic fibrosis as possible predictive measures of outcome of infants having EHBA. METHODS: The outcome of 32 infants with EHBA was correlated to their percutaneous biopsy and postportoenterostomy core liver tissue infiltration by CD4+, CD68+, and CD8+ cells and to the degree of detected fibrosis. RESULTS: Portoenterostomy cores were heavily infiltrated by CD4+, CD8+, and CD68+, compared with the preoperative liver biopsy (P = .008, .004, and .017, respectively). Infants having favorable outcome had more macrophage infiltration in portoenterostomy core compared with those having an unfavorable outcome (25.66 +/- 29.77 per HPF compared with 11.62 +/- 4.58, P = .000). Mean CD4+/CD8+ ratio was 1.54 +/- 1.37 in those who died within 18 months postoperatively and 0.733 +/- 0.48 in others (P = .021). CONCLUSION: Immune-mediated destruction of portal tracts is an integral part of pathogenesis of EHBA.
Authors:
Magd A Kotb; Ahmed El Henawy; Sahaar Talaat; Mona Aziz; Gamal H El Tagy; Mohamed M El Barbary; Wael Mostafa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  40     ISSN:  1531-5037     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-05     Completed Date:  2006-02-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1252-7     Citation Subset:  IM    
Affiliation:
Department of Paediatrics, Cairo University, Cairo, Egypt. malotfi@menanet.net
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Antigens, CD / analysis,  immunology
Antigens, CD4 / analysis
Antigens, CD8 / analysis
Antigens, Differentiation, Myelomonocytic / analysis,  immunology
Bile Ducts, Extrahepatic / immunology*,  pathology
Biliary Atresia / complications*,  immunology*,  surgery
Female
Humans
Immunohistochemistry
Infant
Infant, Newborn
Liver / immunology,  pathology
Liver Cirrhosis, Biliary / etiology,  immunology*,  pathology
Lymphocyte Count
Macrophages / immunology
Male
Portal System / immunology,  pathology
Portoenterostomy, Hepatic
Prognosis
Statistics, Nonparametric
T-Lymphocytes, Cytotoxic / immunology
T-Lymphocytes, Helper-Inducer / immunology
Treatment Outcome
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD4; 0/Antigens, CD8; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 antigen, human

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