| Immune lysis of spin label loaded liposomes incorporating cardiolipin; a new sensitive method for detecting anticardiolipin antibodies in syphilis serology. | |
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MedLine Citation:
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PMID: 321698 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Liposomes prepared from a mixture of the pure lipids cholesterol, lecithin and cardiolipin (molar ratio 50/45/5), are able to bind antibodies directed against Treponema pallidum. When the liposomes are loaded with the water soluble spin label tempocholine chloride, the release of spin label from the liposomes can be monitored directly by observing changes in the paramagnetic resonance (ESR) spectrum from the spin label. The method offers a convenient technique for monitoring the complement-mediated lysis of liposomes, and may be applied in the serological diagnosis of syphilis, and a method for quantitative measurement of complement. |
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Authors:
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E Rosenqvist; A I Vistnes |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of immunological methods Volume: 15 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1977 |
Date Detail:
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Created Date: 1977-05-25 Completed Date: 1977-05-25 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 147-55 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / analysis* Cardiolipins / immunology* Cattle Chickens Complement System Proteins Guinea Pigs Humans Immunologic Techniques* Liposomes / metabolism* Spin Labels* Syphilis Serodiagnosis* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Cardiolipins; 0/Liposomes; 0/Spin Labels; 9007-36-7/Complement System Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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