Document Detail


Immune defense reduces respiratory fitness in Callinectes sapidus, the Atlantic blue crab.
MedLine Citation:
PMID:  16946241     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Crustacean gills function in gas exchange, ion transport, and immune defense against microbial pathogens. Hemocyte aggregates that form in response to microbial pathogens become trapped in the fine vasculature of the gill, leading to the suggestion by others that respiration and ion regulation might by impaired during the course of an immune response. In the present study, injection of the pathogenic bacterium Vibrio campbellii into Callinectes sapidus, the Atlantic blue crab, caused a dramatic decline in oxygen uptake from 4.53 to 2.56 micromol g-1 h-1. This decline in oxygen uptake is associated with a large decrease in postbranchial PO2, from 16.2 (+/-0.46 SEM, n=7) to 13.1 kPa (+/-0.77 SEM, n=9), while prebranchial PO2 remains unchanged. In addition, injection of Vibrio results in the disappearance of a pH change across the gills, an indication of reduced CO2 excretion. The hemolymph hydrostatic pressure change across the gill circulation increases nearly 2-fold in Vibrio-injected crabs compared with a negligible change in pressure across the gill circulation in saline-injected, control crabs. This change, in combination with stability of heart rate and branchial chamber pressure, is indicative of a significant increase in vascular resistance across the gills that is induced by hemocyte nodule formation. A healthy, active blue crab can eliminate most invading bacteria, but the respiratory function of the gills is impaired. Thus, when blue crabs are engaged in the immune response, they are less equipped to engage in oxygen-fueled activities such as predator avoidance, prey capture, and migration. Furthermore, crabs are less fit to invade environments that are hypoxic.
Authors:
Louis E Burnett; Jeremy D Holman; Darwin D Jorgensen; Jennifer L Ikerd; Karen G Burnett
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Biological bulletin     Volume:  211     ISSN:  0006-3185     ISO Abbreviation:  Biol. Bull.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-09-01     Completed Date:  2007-01-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984727R     Medline TA:  Biol Bull     Country:  United States    
Other Details:
Languages:  eng     Pagination:  50-7     Citation Subset:  IM    
Affiliation:
Grice Marine Laboratory, College of Charleston, 205 Fort Johnson, Charleston, South Carolina 29412, USA. BurnettL@cofc.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Brachyura / immunology*,  metabolism*,  microbiology
Gills / metabolism,  microbiology
Hemolymph / chemistry
Oxygen / analysis,  metabolism
Vibrio / metabolism
Water / chemistry
Chemical
Reg. No./Substance:
7732-18-5/Water; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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