Document Detail


Immortalized clones of fibroblastic reticular cells activate virus-specific T cells during virus infection.
MedLine Citation:
PMID:  22550183     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fibroblastic reticular cells (FRCs) are lymphoid stromal cells essential to T-cell migration and survival. Although FRCs are targets of multiple viral infections, little is known about their role during infection due to the cells' scarcity and difficulty in isolating in vivo. To initiate studies of interactions among FRCs, viruses, and immune cells, we isolated and immortalized CD45(-)gp38(+)CD35(-)CD31(-)CD44(+)VCAM1(+) cell lines from C57BL/6 mice designated as immortalized FRC. Using these cloned cell lines, we have established that FRCs express the major histocompatibility complex (MHC) II molecule, a factor necessary for stimulation of CD4(+) T cells thought to be expressed primarily by antigen-presenting cells, along with other T-cell stimulatory ligands in an IFN-γ-dependent manner. In this environment, lymphocytic choriomeningitis virus (LCMV)-infected iFRCs activated naive LCMV-specific CD4(+) and CD8(+) T cells while limiting expansion of effector LCMV-specific T cells. Thus, FRCs effectively presented antigen along with activating signals during viral infection using both MHC I and MHC II molecules, illustrating a previously undescribed interaction with CD4(+) T cells and indicating a unique role for FRCs.
Authors:
Cherie T Ng; Bishnu P Nayak; Christian Schmedt; Michael B A Oldstone
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-05-01
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-16     Completed Date:  2012-07-27     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7823-8     Citation Subset:  IM    
Affiliation:
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen Presentation / immunology*
CD4-Positive T-Lymphocytes / immunology*
Cell Line
Cell Proliferation
Flow Cytometry
Histocompatibility Antigens Class II / immunology*
Interferon-gamma / immunology
Lymphocyte Activation / immunology
Lymphocytic choriomeningitis virus*
Mice
Mice, Inbred C57BL
RNA Virus Infections / immunology*
Reverse Transcriptase Polymerase Chain Reaction
Stromal Cells / immunology*,  virology
Grant Support
ID/Acronym/Agency:
A019484//PHS HHS; HL007195/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Histocompatibility Antigens Class II; 82115-62-6/Interferon-gamma
Comments/Corrections

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