| Immortalization with telomerase of the Nestin-positive cells of the human pancreas. | |
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MedLine Citation:
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PMID: 12589817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells expressing the neuronal stem cell marker Nestin are present in the human pancreas but the biological role of these cells has yet to be resolved. We report here the establishment with the catalytic subunit of human telomerase (hTERT) of a line of normal human cells representing this cell type. Primary human cells derived from the ducts of the pancreas were transduced with an hTERT cDNA. The infected cells became positive for telomerase, failed to senesce, and were still proliferating after more than 150 doublings. The immortalized cells were positive for the expression of Nestin (at both the mRNA and protein levels) and were found to be free of cancer-associated changes: diploid and expressing wild type p16(INK4a), p53, and K-Ras. An established line of normal human cells representing this cell type should be of great value to help define the biological properties of this novel cell type. |
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Authors:
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K M Lee; C Nguyen; A B Ulrich; P M Pour; M M Ouellette |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 301 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-02-18 Completed Date: 2003-04-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 1038-44 Citation Subset: IM |
Affiliation:
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Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Catalytic Domain Cell Line, Transformed Cell Survival Cell Transformation, Neoplastic DNA Primers / genetics DNA-Binding Proteins Genes, p16 Genes, p53 Humans Intermediate Filament Proteins / genetics, metabolism* Nerve Tissue Proteins* Neurons / cytology, metabolism Pancreatic Ducts / cytology*, metabolism* RNA, Messenger / genetics, metabolism Stem Cells / cytology, metabolism Telomerase / genetics*, metabolism Transduction, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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P50 CA 72712-03S2/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA-Binding Proteins; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/nestin; EC 2.7.7.49/Telomerase |
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