Document Detail

Immortalization of normal human gingival keratinocytes and cytological and cytogenetic characterization of the cells.
MedLine Citation:
PMID:  19184294     Owner:  NLM     Status:  MEDLINE    
Most in vitro studies of oral carcinogenesis in human cells are carried out with oral keratinocytes immortalized by human papillomavirus type 16 DNA. However, because various etiological factors for oral cancer are known, it is important to establish new human keratinocyte cell lines useful for studying the mechanism of oral carcinogenesis. Normal human gingival keratinocytes in secondary cultures grown in serum-free medium were either transfected with origin (-) SV40 DNA or sequentially transfected with origin (-) SV40 DNA and human c-fos. The transfected cells were continually passaged and analyzed for cytological and cytogenetic characterizations. Four immortal cell lines were grown for over 1100 days in culture and maintained a vigorous growth for over 250 population doublings. They expressed SV40 T antigen, cytokeratins 8 and 18, and E-cadherin, and overexpressed the c-Fos protein. The immortal cell lines had telomerase activity but lacked transformed phenotypes on soft agar or in nude mice. Each cell line had nonrandom chromosomal abnormalities and minisatellite alterations. One of the immortal cell lines, NDUSD-1, retained the capability to deposit calcium, which was also demonstrated in normal human gingival keratinocytes by alizarin red staining, indicating the possibility that NDUSD-1 cells may retain some natural characteristics of normal gingival keratinocytes. Because the oral ectoderm plays an important role in tooth development, these immortal cell lines may be useful in various experimental models for investigations of oral biology and oral carcinogenesis.
Chikahiro Kubo; Takeo W Tsutsui; Yukiko Tamura; Shin-Ichi Kumakura; Takeki Tsutsui
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Publication Detail:
Type:  Journal Article     Date:  2009-01-29
Journal Detail:
Title:  Odontology / the Society of the Nippon Dental University     Volume:  97     ISSN:  1618-1247     ISO Abbreviation:  Odontology     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-06-17     Revised Date:  2013-09-12    
Medline Journal Info:
Nlm Unique ID:  101134822     Medline TA:  Odontology     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  18-31     Citation Subset:  D; IM    
Department of Pharmacology, The Nippon Dental University, School of Life Dentistry at Tokyo, Tokyo, Japan.
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MeSH Terms
Anthraquinones / diagnostic use
Antigens, Polyomavirus Transforming / analysis
Cadherins / analysis
Calcium / metabolism
Cell Line
Cell Line, Transformed
Cell Proliferation
Cell Transformation, Neoplastic / genetics,  pathology*
Cell Transformation, Viral / genetics
Chromosome Aberrations
Coloring Agents / diagnostic use
DNA, Viral / genetics
Genes, fos / genetics
Gingiva / cytology*
Keratin-18 / analysis
Keratin-8 / analysis
Keratinocytes / cytology*,  pathology
Mice, Nude
Minisatellite Repeats / genetics
Proto-Oncogene Proteins c-fos / analysis
Simian virus 40 / genetics,  immunology
Telomerase / metabolism
Time Factors
Transfection / methods
Reg. No./Substance:
0/Anthraquinones; 0/Antigens, Polyomavirus Transforming; 0/Cadherins; 0/Coloring Agents; 0/DNA, Viral; 0/Keratin-18; 0/Keratin-8; 0/Proto-Oncogene Proteins c-fos; 60MEW57T9G/alizarin; 7440-70-2/Calcium; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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