Document Detail


Immortalization of normal adult human middle ear epithelial cells using a retrovirus containing the E6/E7 genes of human papillomavirus type 16.
MedLine Citation:
PMID:  12090706     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A human middle ear epithelial cell line (HMEEC-1) was established using human papillomavirus E6/E7 genes. HMEEC-1 has remained morphologically and phenotypically stable, even after 50 passages. The cells are anchorage-dependent and nontumorigenic when injected into nude mice. This cell line thus provides a new tool for the study of normal cell biology and the pathological processes associated with the epithelial cells of the middle ear in otitis media. HMEEC-1 will also be useful in the search for new drugs and biological agents for the treatment of otitis media.
Authors:
Young-Myoung Chun; Sung-Kyun Moon; Haa-Yung Lee; Paul Webster; Derald E Brackmann; Johng S Rhim; David J Lim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Annals of otology, rhinology, and laryngology     Volume:  111     ISSN:  0003-4894     ISO Abbreviation:  Ann. Otol. Rhinol. Laryngol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-07-01     Completed Date:  2002-07-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0407300     Medline TA:  Ann Otol Rhinol Laryngol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  507-17     Citation Subset:  AIM; IM    
Affiliation:
Gonda Department of Cell and Molecular Biology, Los Angeles, California, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Cell Line
Ear, Middle / cytology*
Epithelial Cells
Genetic Vectors
Humans
Mice
Mice, Nude
Oncogene Proteins, Viral / genetics
Papillomaviridae / genetics*
Repressor Proteins*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/E6 protein, Human papillomavirus type 16; 0/Oncogene Proteins, Viral; 0/Repressor Proteins; 0/oncogene protein E7, Human papillomavirus type 16

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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