| Immortalization and characterization of porcine ventricular endocardial endothelial cells. | |
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MedLine Citation:
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PMID: 17364895 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endocardial endothelial cells (EECs), which form the inner lining of the cavities of the heart, are a distinct cell population whose dysfunction can be critical in pathological conditions of heart. Insights into the role and organization of these cells in pathological states of the heart are limited mainly due to a dearth of experimental models. To date no endocardial endothelial cell line is available. The authors attempted to immortalize porcine ventricular EECs by transfecting the cells with human telomerase reverse transcriptase (hTERT). EECs immortalized by ectopic expression of hTERT exhibit phenotypic and functional characteristics similar to primary EECs. The EE cell line could be useful for the study of mechanisms involved in the interaction of EECs with the underlying myocardium and cardiac interstitium and as useful tools in understanding their role in diseased states of heart. |
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Authors:
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Leena Kuruvilla; Santhoshkumar T R; Chandrasekharan Cheranellore Kartha |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endothelium : journal of endothelial cell research Volume: 14 ISSN: 1062-3329 ISO Abbreviation: Endothelium Publication Date: 2007 Jan-Feb |
Date Detail:
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Created Date: 2007-03-16 Completed Date: 2007-06-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9412590 Medline TA: Endothelium Country: United States |
Other Details:
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Languages: eng Pagination: 35-43 Citation Subset: IM |
Affiliation:
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Division of Cellular and Molecular Cardiology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Cell Division / physiology Cell Line, Transformed Endocardium / cytology* Endothelial Cells / cytology*, metabolism* Endothelium, Vascular / cytology* Fluorescein-5-isothiocyanate Fluorescent Antibody Technique, Direct Fluorescent Dyes Heart Ventricles / cytology* Humans Immunohistochemistry Kinetics Nitrites / analysis Reverse Transcriptase Polymerase Chain Reaction Swine Telomerase / analysis, genetics, metabolism* Transfection |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Nitrites; 3326-32-7/Fluorescein-5-isothiocyanate; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase |
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