Document Detail

Immortalization and characterization of porcine ventricular endocardial endothelial cells.
MedLine Citation:
PMID:  17364895     Owner:  NLM     Status:  MEDLINE    
Endocardial endothelial cells (EECs), which form the inner lining of the cavities of the heart, are a distinct cell population whose dysfunction can be critical in pathological conditions of heart. Insights into the role and organization of these cells in pathological states of the heart are limited mainly due to a dearth of experimental models. To date no endocardial endothelial cell line is available. The authors attempted to immortalize porcine ventricular EECs by transfecting the cells with human telomerase reverse transcriptase (hTERT). EECs immortalized by ectopic expression of hTERT exhibit phenotypic and functional characteristics similar to primary EECs. The EE cell line could be useful for the study of mechanisms involved in the interaction of EECs with the underlying myocardium and cardiac interstitium and as useful tools in understanding their role in diseased states of heart.
Leena Kuruvilla; Santhoshkumar T R; Chandrasekharan Cheranellore Kartha
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endothelium : journal of endothelial cell research     Volume:  14     ISSN:  1062-3329     ISO Abbreviation:  Endothelium     Publication Date:    2007 Jan-Feb
Date Detail:
Created Date:  2007-03-16     Completed Date:  2007-06-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9412590     Medline TA:  Endothelium     Country:  United States    
Other Details:
Languages:  eng     Pagination:  35-43     Citation Subset:  IM    
Division of Cellular and Molecular Cardiology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
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MeSH Terms
Blotting, Western
Cell Division / physiology
Cell Line, Transformed
Endocardium / cytology*
Endothelial Cells / cytology*,  metabolism*
Endothelium, Vascular / cytology*
Fluorescent Antibody Technique, Direct
Fluorescent Dyes
Heart Ventricles / cytology*
Nitrites / analysis
Reverse Transcriptase Polymerase Chain Reaction
Telomerase / analysis,  genetics,  metabolism*
Reg. No./Substance:
0/Fluorescent Dyes; 0/Nitrites; 3326-32-7/Fluorescein-5-isothiocyanate; EC protein, human; EC

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