| Immortalization and characterization of a nociceptive dorsal root ganglion sensory neuronal line. | |
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MedLine Citation:
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PMID: 17565537 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Development of neuroprotective strategies for peripheral neuropathies requires high-throughput drug screening assays with appropriate cell types. Currently, immortalized dorsal root ganglion (DRG) sensory neuronal cell lines that maintain nociceptive sensory neuronal properties are not available. We generated immortalized DRG neuronal lines from embryonic day 14.5 rats. Here, we show that one of the immortalized DRG neuronal lines, 50B11, has the properties of a nociceptive neuron. When differentiated in the presence of forskolin, these cells extend long neurites, express neuronal markers, and generate action potentials. They express receptors and markers of small-diameter sensory neurons and upregulate appropriate receptor populations when grown in the presence of glial cell line-derived neurotrophic factor or nerve growth factor. Furthermore, they express capsaicin receptor transient receptor potential vanilloid family-1 (TRPV-1) and respond to capsaicin with increases in intracellular calcium. In a 96-well plate format, these neurons show a decline in ATP levels when exposed to dideoxycytosine (ddC) in a proper time- and dose-dependent manner. This ddC-induced reduction in ATP levels correlates with axonal degeneration. The immortalized DRG neuronal cell line 50B11 can be used for high-throughput drug screening for neuroprotective agents for axonal degeneration and antinociceptive drugs that block TRPV-1. |
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Authors:
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Weiran Chen; Ruifa Mi; Norman Haughey; Murat Oz; Ahmet Höke |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of the peripheral nervous system : JPNS Volume: 12 ISSN: 1085-9489 ISO Abbreviation: J. Peripher. Nerv. Syst. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-13 Completed Date: 2007-09-26 Revised Date: 2013-03-07 |
Medline Journal Info:
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Nlm Unique ID: 9704532 Medline TA: J Peripher Nerv Syst Country: United States |
Other Details:
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Languages: eng Pagination: 121-30 Citation Subset: IM |
Affiliation:
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Department of Neurology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Polyomavirus Transforming / genetics Cell Differentiation Cell Line* Drug Evaluation, Preclinical Electroporation Ganglia, Spinal / cytology* Gene Expression Genetic Vectors Humans Lentivirus / genetics Neurons / cytology*, physiology* Neuroprotective Agents / pharmacology Nociceptors / cytology*, physiology* Polymerase Chain Reaction Rats TRPV Cation Channels / biosynthesis Telomerase / genetics |
| Grant Support | |
ID/Acronym/Agency:
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P01MH70056/MH/NIMH NIH HHS; P30 MH075673/MH/NIMH NIH HHS; R01 AG023471/AG/NIA NIH HHS; R01NS43991/NS/NINDS NIH HHS; R01NS47972/NS/NINDS NIH HHS; R21 MH072534/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Polyomavirus Transforming; 0/Neuroprotective Agents; 0/TRPV Cation Channels; 0/Trpv1 protein, rat; EC 2.7.7.49/Telomerase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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