Document Detail


Immortalization and characterization of a nociceptive dorsal root ganglion sensory neuronal line.
MedLine Citation:
PMID:  17565537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Development of neuroprotective strategies for peripheral neuropathies requires high-throughput drug screening assays with appropriate cell types. Currently, immortalized dorsal root ganglion (DRG) sensory neuronal cell lines that maintain nociceptive sensory neuronal properties are not available. We generated immortalized DRG neuronal lines from embryonic day 14.5 rats. Here, we show that one of the immortalized DRG neuronal lines, 50B11, has the properties of a nociceptive neuron. When differentiated in the presence of forskolin, these cells extend long neurites, express neuronal markers, and generate action potentials. They express receptors and markers of small-diameter sensory neurons and upregulate appropriate receptor populations when grown in the presence of glial cell line-derived neurotrophic factor or nerve growth factor. Furthermore, they express capsaicin receptor transient receptor potential vanilloid family-1 (TRPV-1) and respond to capsaicin with increases in intracellular calcium. In a 96-well plate format, these neurons show a decline in ATP levels when exposed to dideoxycytosine (ddC) in a proper time- and dose-dependent manner. This ddC-induced reduction in ATP levels correlates with axonal degeneration. The immortalized DRG neuronal cell line 50B11 can be used for high-throughput drug screening for neuroprotective agents for axonal degeneration and antinociceptive drugs that block TRPV-1.
Authors:
Weiran Chen; Ruifa Mi; Norman Haughey; Murat Oz; Ahmet Höke
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of the peripheral nervous system : JPNS     Volume:  12     ISSN:  1085-9489     ISO Abbreviation:  J. Peripher. Nerv. Syst.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-13     Completed Date:  2007-09-26     Revised Date:  2013-03-07    
Medline Journal Info:
Nlm Unique ID:  9704532     Medline TA:  J Peripher Nerv Syst     Country:  United States    
Other Details:
Languages:  eng     Pagination:  121-30     Citation Subset:  IM    
Affiliation:
Department of Neurology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Polyomavirus Transforming / genetics
Cell Differentiation
Cell Line*
Drug Evaluation, Preclinical
Electroporation
Ganglia, Spinal / cytology*
Gene Expression
Genetic Vectors
Humans
Lentivirus / genetics
Neurons / cytology*,  physiology*
Neuroprotective Agents / pharmacology
Nociceptors / cytology*,  physiology*
Polymerase Chain Reaction
Rats
TRPV Cation Channels / biosynthesis
Telomerase / genetics
Grant Support
ID/Acronym/Agency:
P01MH70056/MH/NIMH NIH HHS; P30 MH075673/MH/NIMH NIH HHS; R01 AG023471/AG/NIA NIH HHS; R01NS43991/NS/NINDS NIH HHS; R01NS47972/NS/NINDS NIH HHS; R21 MH072534/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Neuroprotective Agents; 0/TRPV Cation Channels; 0/Trpv1 protein, rat; EC 2.7.7.49/Telomerase
Comments/Corrections

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