| Immortalization and characterization of human dental pulp cells with odontoblastic differentiation. | |
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MedLine Citation:
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PMID: 17350587 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To immortalize human dental pulp (HDP) cell showing stable growth and high mineralization activities in vitro. DESIGN: HDP cells were obtained from a healthy third molar and immortalized by transfection with human telomerase transcriptase (hTERT) gene. To examine the characters of hTERT transfected HDP (HDP-hTERT) cells, we examined expression of mRNA for dentin sialophosphoprotein (DSSP), type I collagen (COLI), alkaline phosphatase (ALP) and bone sialoprotein (BSP) by RT-PCR. In addition, we examined ALP activity by biochemical method and nodule formation by alizarin red S (ALZ) staining. RESULTS: HDP-hTERT was obtained by transfection with hTERT gene. These cells bypassed the senescence and grew over 120 population doublings (PDLs) without significant growth retardation. High expression of hTERT was confirmed in HDP-hTERT by RT-PCR and showed remarkable telomerase activity. Both HDP-original (HDP-ori) and HDP-hTERT expressed DSSP, COLI, ALP and BSP mRNA and showed ALP activity and ALZ staining at the same levels. CONCLUSIONS: We were able to establish a cell line of immortalized human dental pulp cells with odontoblastic differentiation which will be a useful cell model for studying the mechanism of proliferation and differentiation of odontoblasts. |
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Authors:
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Masae Kitagawa; Hirota Ueda; Sinji Iizuka; Kiyako Sakamoto; Hiroko Oka; Yasusei Kudo; Ikuko Ogawa; Mutsumi Miyauchi; Hidetoshi Tahara; Takashi Takata |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-12 |
Journal Detail:
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Title: Archives of oral biology Volume: 52 ISSN: 0003-9969 ISO Abbreviation: Arch. Oral Biol. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-06-18 Completed Date: 2007-12-18 Revised Date: 2010-02-05 |
Medline Journal Info:
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Nlm Unique ID: 0116711 Medline TA: Arch Oral Biol Country: England |
Other Details:
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Languages: eng Pagination: 727-31 Citation Subset: D; IM |
Affiliation:
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Department of Oral and Maxillofacial Pathobiology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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analysis Anthraquinones / diagnostic use Biological Markers / analysis Cell Differentiation / genetics Cell Line, Transformed Cell Proliferation Collagen Type I / analysis Coloring Agents / diagnostic use Dental Pulp / cytology* Extracellular Matrix Proteins / analysis Genetic Vectors / genetics Humans Odontoblasts / cytology* Phosphoproteins / analysis Retroviridae / genetics Sialoglycoproteins / analysis Telomerase / genetics Transfection / methods Transformation, Genetic / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Anthraquinones; 0/Biological Markers; 0/Collagen Type I; 0/Coloring Agents; 0/Extracellular Matrix Proteins; 0/Phosphoproteins; 0/Sialoglycoproteins; 0/dentin sialophosphoprotein; 0/integrin-binding sialoprotein; 83-61-4/Alizarin Red S; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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