Document Detail


Immortalization and characterization of human dental pulp cells with odontoblastic differentiation.
MedLine Citation:
PMID:  17350587     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To immortalize human dental pulp (HDP) cell showing stable growth and high mineralization activities in vitro. DESIGN: HDP cells were obtained from a healthy third molar and immortalized by transfection with human telomerase transcriptase (hTERT) gene. To examine the characters of hTERT transfected HDP (HDP-hTERT) cells, we examined expression of mRNA for dentin sialophosphoprotein (DSSP), type I collagen (COLI), alkaline phosphatase (ALP) and bone sialoprotein (BSP) by RT-PCR. In addition, we examined ALP activity by biochemical method and nodule formation by alizarin red S (ALZ) staining. RESULTS: HDP-hTERT was obtained by transfection with hTERT gene. These cells bypassed the senescence and grew over 120 population doublings (PDLs) without significant growth retardation. High expression of hTERT was confirmed in HDP-hTERT by RT-PCR and showed remarkable telomerase activity. Both HDP-original (HDP-ori) and HDP-hTERT expressed DSSP, COLI, ALP and BSP mRNA and showed ALP activity and ALZ staining at the same levels. CONCLUSIONS: We were able to establish a cell line of immortalized human dental pulp cells with odontoblastic differentiation which will be a useful cell model for studying the mechanism of proliferation and differentiation of odontoblasts.
Authors:
Masae Kitagawa; Hirota Ueda; Sinji Iizuka; Kiyako Sakamoto; Hiroko Oka; Yasusei Kudo; Ikuko Ogawa; Mutsumi Miyauchi; Hidetoshi Tahara; Takashi Takata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-12
Journal Detail:
Title:  Archives of oral biology     Volume:  52     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-06-18     Completed Date:  2007-12-18     Revised Date:  2010-02-05    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  727-31     Citation Subset:  D; IM    
Affiliation:
Department of Oral and Maxillofacial Pathobiology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / analysis
Anthraquinones / diagnostic use
Biological Markers / analysis
Cell Differentiation / genetics
Cell Line, Transformed
Cell Proliferation
Collagen Type I / analysis
Coloring Agents / diagnostic use
Dental Pulp / cytology*
Extracellular Matrix Proteins / analysis
Genetic Vectors / genetics
Humans
Odontoblasts / cytology*
Phosphoproteins / analysis
Retroviridae / genetics
Sialoglycoproteins / analysis
Telomerase / genetics
Transfection / methods
Transformation, Genetic / genetics*
Chemical
Reg. No./Substance:
0/Anthraquinones; 0/Biological Markers; 0/Collagen Type I; 0/Coloring Agents; 0/Extracellular Matrix Proteins; 0/Phosphoproteins; 0/Sialoglycoproteins; 0/dentin sialophosphoprotein; 0/integrin-binding sialoprotein; 83-61-4/Alizarin Red S; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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