| Immortalization of ameloblastoma cells via reactivation of telomerase function: Phenotypic and molecular characteristics. | |
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MedLine Citation:
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PMID: 19833545 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Ameloblastoma (AM) is recognized as a benign tumour but locally invasive with a high risk of recurrence. In vitro model systems for studying AM are limited due to the fact that AM cells grow poorly and begin to senesce early. Japanese researchers have reported the construction of an AM cell line, AM-1, by exposing cells to human papillomavirus 16 (HPV16) but retaining the potential of transformation. In this study, we used a retroviral infection method to over-express the human telomerase reverse transcriptase (hTERT) gene to acquire immortality of hTERT(+)-AM cells. Furthermore, it was revealed both by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot that the pathway of immortalization was loss of p16, not p53 or p21. Also, there was no evidence indicating that the hTERT(+)-AM cells underwent malignant transformation by the nude mouse tumorigenicity assay. Taken together, this hTERT-immortalized cell line may be a potentially valuable and reliable cell model for further study of the invasive properties of AM in vitro. |
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Authors:
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Qian Tao; Biao Lv; Bin Qiao; Chao-qun Zheng; Zhi-feng Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-14 |
Journal Detail:
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Title: Oral oncology Volume: 45 ISSN: 1368-8375 ISO Abbreviation: Oral Oncol. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9709118 Medline TA: Oral Oncol Country: England |
Other Details:
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Languages: eng Pagination: e239-44 Citation Subset: IM |
Affiliation:
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Department of Oral and maxillofacial Surgery, Guanghua School and Hospital of Stomatology and Institute of Stomatological Research, Sun Yat-sen University, Guangzhou 510055, China. taoqian@mail.sysu.edu.cn |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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