| Immortal phenotype of the esophageal epithelial cells in the process of immortalization. | |
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MedLine Citation:
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PMID: 12373308 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To search for potential biomarkers used to monitor the process of immortalization, we investigated the relative level of telomerase activity and other immortal phenotypes in the SHEE esophageal epithelial cell line. This human fetal esophageal epithelial cell line, induced by human papilloma virus (HPV) 18 E6E7, was continually propagated over 100 passages. Fourteenth passage cells (SHEE14) were cultured in a flask with a serum-free medium and continually cultured to the 30th passage (SHEE30). Cells of SHEE14, SHEE20 and SHEE30 were examined according to cell morphology, cell cycle, apoptosis, contact-inhibition growth, anchorage- dependency, dose-dependency to epithelial growth factors (EGF), telomerase activity and tumorigenicity. The SHEE14 cells exhibited good differentiation with contact-inhibition and anchorage-dependent growth. The SHEE20 cells exhibited increase of senescent and apoptotic cells, and difficulty in propagation. The SHEE30 cells exhibited a higher proliferative index and some undifferentiated cells, with weakened contact-inhibition and anchorage-dependent growth. The telomerase was activated in cells of SHEE30, but not in SHEE14 and SHEE20 cells. The different response to dose-dependency to EGF was not statistically different in SHEE14 and SHEE30. Three groups of cells displayed lack of tumor formation in nude mice. Compared with SHEE14 and SHEE20, SHEE30 cells were of immortalized status with immortal phenotype, which consisted of telomerase activity, increase of cell proliferation, weakened contact-inhibition and anchorage-dependent growth, dose dependency to EGF and lack of tumor formation. From passage 14 to 30th passage, SHEE cells went through cellular senescence, apoptosis and immortalization. With a view toward diagnostic and biological aspects, telomerase activity is a crucial step and a cardinal requirement for immortalization. The telomerase activity and other immortal phenotypes are potential markers for monitoring the process of immortalization. |
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Authors:
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Zhong-Ying Shen; Li-Yan Xu; En-Min Li; Jian Shen; Rui-Ming Zheng; Wei-Jia Cai; Yi Zeng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of molecular medicine Volume: 10 ISSN: 1107-3756 ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-10-09 Completed Date: 2002-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 641-6 Citation Subset: IM |
Affiliation:
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Department of Pathology, Medical College of Shantou University, Shantou 515031, PR China. zhongyingshen@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers Cell Aging Cell Cycle Cell Division Cell Line Cell Transformation, Viral Cell Transplantation Colony-Forming Units Assay Contact Inhibition Epidermal Growth Factor / administration & dosage Epithelial Cells / cytology, drug effects, enzymology Esophagus / cytology*, enzymology Fetus / cytology Humans Mice Mice, Inbred BALB C Mice, Nude Papillomaviridae Phenotype Telomerase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 62229-50-9/Epidermal Growth Factor; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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