Document Detail


Immobilized culture and transfection microarray of non-adherent cells.
MedLine Citation:
PMID:  21104061     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Cell-based microarrays are promising tools for high-throughput functional analysis of gene products, but their application has been limited to adherent cells due to the difficulty in immobilization of non-adherent cells. Herein, we have introduced our techniques that can rapidly and strongly immobilize non-adherent cells and can allow the transfection of non-adherent cells with plasmid cDNA and small interfering RNA (siRNA) at a defined position on substrates with poly(ethylene-glycol)-lipid-modified surface.
Authors:
Satoshi Yamaguchi; Erika Matsunuma; Teruyuki Nagamune
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  706     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2010-11-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  151-7     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan. yamaguchi@bio.t.u-tokyo.ac.jp
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