Document Detail


Immobilization of uricase within polystyrene using polymaleimidostyrene as a stabilizer and its application to uric acid sensor.
MedLine Citation:
PMID:  17386751     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel amperometric uric acid (UA) sensor has been developed by coating the surface of a gold electrode with a polystyrene (PS) membrane formed by 30 microL of a 30 mg mL(-1) PS chloroform solution combined with 30 microL of a 5 mg mL(-1) polymaleimidostyrene (PMS) solution as a dispersant for enzyme, uricase; this membrane has been successfully employed as an immobilization support for uricase. In the PS membrane, PMS forms micelle-like structures containing uricase in an active state. This immobilized uricase membrane permits the permeation of oxygen, which is consumed by the uricase reaction. A good linear relationship is obtained over the concentration range of 5-105 microM. The concentration of uric acid was determined at a negative potential based on the decrease in the reduction current of oxygen and the interference of l-ascorbic acid can be completely eliminated.
Authors:
Xiuyun Wang; Tokio Hagiwara; Shunichi Uchiyama
Publication Detail:
Type:  Journal Article     Date:  2007-01-19
Journal Detail:
Title:  Analytica chimica acta     Volume:  587     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-27     Completed Date:  2007-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  41-6     Citation Subset:  IM    
Affiliation:
Department of Materials & Science, Graduate School of Engineering, Saitama Institute of Technology, 1690 Fusaiji Fukaya, Saitama 369-0293, Japan.
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MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / chemistry
Biosensing Techniques*
Chloroform / chemistry*
Dose-Response Relationship, Drug
Electrochemistry / methods*
Enzymes, Immobilized / chemistry*
Maleimides / chemistry*
Micelles
Microscopy, Electron, Scanning
Models, Chemical
Oxygen / chemistry
Polystyrenes / chemistry*
Urate Oxidase / analysis,  chemistry*
Uric Acid / chemistry*
Yeasts / enzymology
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Maleimides; 0/Micelles; 0/Polystyrenes; 0/polymaleimidostyrene; 50-81-7/Ascorbic Acid; 67-66-3/Chloroform; 69-93-2/Uric Acid; 7782-44-7/Oxygen; EC 1.7.3.3/Urate Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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