Document Detail


Immobilization of uricase in Langmuir and Langmuir-Blodgett films of fatty acids and a possible uric acid colorimetric sensor.
MedLine Citation:
PMID:  21875709     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Preserving the enzyme structure in solid films is key for producing various bioelectronic devices, including biosensors, which has normally been performed with nanostructured films that allow for control of molecular architectures. In this paper, we investigate the adsorption of uricase onto Langmuir monolayers of stearic acid (SA), and their transfer to solid supports as Langmuir-Blodgett (LB) films. Structuring of the enzyme in β-sheets was preserved in the form of 1-layer LB film, which was corroborated with a higher catalytic activity than for other uricase-containing LB film architectures where the β-sheets structuring was not preserved. The optimized architecture was also used to detect uric acid within a range covering typical concentrations in the human blood. The approach presented here not only allows for an optimized catalytic activity toward uric acid but also permits one to explain why some film architectures exhibit a superior performance.
Authors:
Nathaly C M Zanon; Osvaldo N Oliveira; Luciano Caseli
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-16
Journal Detail:
Title:  Journal of colloid and interface science     Volume:  -     ISSN:  1095-7103     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0043125     Medline TA:  J Colloid Interface Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Instituto de Ciências Ambientais, Química e Farmacêuticas, Universidade Federal de São Paulo, Diadema, SP, Brazil.
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