Document Detail


Immobilization of trypsin onto "molded" macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) rods and use of the conjugates as bioreactors and for affinity chromatography.
MedLine Citation:
PMID:  18623589     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Trypsin immobilization onto continuous "molded" rods of porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) and some applications of the conjugate have been studied. The rods polymerized within a tubular mold (chromatographic column), were treated in situ with ethylenediamine, activated with glutaraldehyde and finally modified with trypsin. The performance of the trypsin-modified rods was evaluated and compared to that of poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads, modified with the same enzyme. Overall the enzyme-modified rods performed substantially better than the corresponding beads. In particular, the performance of the molded supports as enzymatic reactors or as chromatographic media benefits greatly from the enhanced mass transfer that is characteristic of the molded rod at high flow rates. (c) 1996 John Wiley & Sons, Inc.
Authors:
M Petro; F Svec; J M Fréchet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  49     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  2008-07-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  355-63     Citation Subset:  -    
Affiliation:
Cornell University, Department of Chemistry, Baker Laboratory, Ithaca, New York 14853-1301.
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