Document Detail

Immobilization of thrombocytes on PCL nanofibres enhances chondrocyte proliferation in vitro.
MedLine Citation:
PMID:  21401760     Owner:  NLM     Status:  In-Data-Review    
Objectives:  The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cell-seeded scaffold implants a one-step intervention. Materials and methods:  We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Immobilized, these thrombocytes attached to nanofibre scaffolds were used as a nanoscale delivery system for native (autologous) proliferation and differentiation factors, in vitro. Pig chondrocytes were seeded on the thrombocyte-coated scaffolds and levels of proliferation and differentiation of these cells were compared with those seeded on non-coated scaffolds. Results:  Immobilized thrombocytes on PCL nanofibres effectively enhanced chondrocyte proliferation due to time-dependent degradation of thrombocytes and release of their GFs. Conclusions:  These simply functionalized scaffolds present new possibilities for nanofibre applications, as smart cell scaffolds equipped with a GF delivery tool.
R Jakubova; A Mickova; M Buzgo; M Rampichova; E Prosecka; D Tvrdik; E Amler
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell proliferation     Volume:  44     ISSN:  1365-2184     ISO Abbreviation:  Cell Prolif.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  England    
Other Details:
Languages:  eng     Pagination:  183-91     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic Institute of Pathology, First Faculty of Medicine and General Teaching Hospital, Charles University, Prague, Czech Republic Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.
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