Document Detail


Immobilization of subtilisin on polycaprolactam for antimicrobial food packaging applications.
MedLine Citation:
PMID:  21910484     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Subtilisin was immobilized on polycaprolactam and used for food packaging applications in order to reduce the transference of microorganism from the packaging material to the packaged food material. Optimized condition for subtilisin immobilization was pH - 8, temperature - 4 °C, Glutaraldehyde - 0.5%, incubation time - 25 h and subtilisin concentration - 600 μL. Formation of -CH=N- at 1576 cm-1 in the Fourier Transform Infra Red (FTIR) spectrum confirmed the immobilization. Subtilisin immobilized polycaprolactam (SIP) exhibited highest residual activity of 106.67±4.41 and 104.67±0.88% at 40° C and pH 8 respectively and retained residual activity of 94% at the end of 56 days when compared to 21.33±4.10% in the case of free subtilisin. SIP significantly (p<0.05) lowered the CFU, dry weight, protein and carbohydrate content in bacterial and fungal biofilm. Practical application of the SIP on ham steaks at 4 and 20 °C showed a 2 to 3 times reduction of Staphylococcus aureus as well as Escherichia coli cells in the range of p<0.05.
Authors:
Prabhawathi Veluchamy; Ponnurengam Malliapaan Sivakumar; Mukesh Doble
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-12
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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