| Immobilization of the soluble domain of human complement receptor 1 on agarose-encapsulated islets for the prevention of complement activation. | |
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MedLine Citation:
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PMID: 20739058 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The transplantation of islets of Langerhans has been successfully applied to the treatment of insulin-dependent diabetes. However, a shortage of human donors is the hardest obstacle to overcome. We aimed to develop a bioartificial pancreas that can realize xeno-islet transplantation. The islets were encapsulated in agarose microbeads carrying the soluble domain of human complement receptor 1 (sCR1), which is an effective inhibitor of the classical and alternative complement activation pathways. When naked rat islets were cultured in rabbit serum, large amounts of insulin leaked from the damaged islets over the course of a few days incubation, but no damaged cells were observed among islets in sCR1-agarose microbeads cultured in rabbit serum for 4 days. Although low levels of insulin were detected in the rabbit serum, the insulin did not leak from damaged β-cells, it was physiological insulin secreted by the β-cells. |
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Authors:
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Nguyen Minh Luan; Yuji Teramura; Hiroo Iwata |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-24 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-10-04 Completed Date: 2011-01-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 8847-53 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-Cho, Shogoin, Sakyo-Ku, Kyoto 606-8507, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells Complement Activation* / drug effects Cricetinae Cricetulus Glucose / metabolism Haptens / immunology Humans Hydrogel / chemistry Immobilized Proteins / chemistry*, metabolism* Insulin / secretion Islets of Langerhans / drug effects, metabolism*, secretion Liposomes / metabolism Protein Structure, Tertiary Rabbits Rats Receptors, Complement / chemistry*, isolation & purification, metabolism* Sepharose / chemistry*, pharmacology Solubility / drug effects Sulfhydryl Compounds / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Haptens; 0/Immobilized Proteins; 0/Liposomes; 0/Receptors, Complement; 0/Sulfhydryl Compounds; 11061-68-0/Insulin; 25852-47-5/Hydrogel; 50-99-7/Glucose; 9012-36-6/Sepharose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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