| Immobilization of recombinant thermostable beta-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk. | |
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MedLine Citation:
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PMID: 19164659 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A recombinant thermostable beta-galactosidase from Bacillus stearothermophilus was immobilized onto chitosan using Tris(hydroxymethyl)phosphine (THP) and glutaraldehyde, and a packed bed reactor was utilized to hydrolyze lactose in milk. The thermostability and enzyme activity of THP-immobilized beta-galactosidase during storage was superior to that of free and glutaraldehyde-immobilized enzymes. The THP-immobilized beta-galactosidase showed greater relative activity in the presence of Ca(2+) than the free enzyme and was stable during the storage at 4 degrees C for 6 wk, whereas the free enzyme lost 31% of the initial activity under the same storage conditions. More than 80% of lactose hydrolysis in milk was achieved after 2 h of operation in the reactor. Therefore, THP-immobilized recombinant thermostable beta-galactosidase from Bacillus stearothermophilus has the potential for application in the production of lactose-hydrolyzed milk. |
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Authors:
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W Chen; H Chen; Y Xia; J Yang; J Zhao; F Tian; H P Zhang; H Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of dairy science Volume: 92 ISSN: 1525-3198 ISO Abbreviation: J. Dairy Sci. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-23 Completed Date: 2009-02-09 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 491-8 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China. weichen@jiangnan.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Food Handling Geobacillus stearothermophilus / enzymology* Hydrogen-Ion Concentration Hydrolysis* Ions / metabolism Lactose / metabolism* Metals / metabolism Milk / chemistry* Recombinant Proteins / metabolism* Temperature Time Factors beta-Galactosidase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Ions; 0/Metals; 0/Recombinant Proteins; 63-42-3/Lactose; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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