Document Detail

Immobilization of catalase by entrapment of permeabilized yeast cells in hen egg white using glutaraldehyde.
MedLine Citation:
PMID:  15134907     Owner:  NLM     Status:  MEDLINE    
An immobilized preparation of whole cell-based catalase was obtained by cross-linking the yeast cells permeabilized with toluene in hen egg white using glutaraldehyde. Optimal preparations were obtained when cross-linking was carried out for 2 h at 4 degrees C. Immobilized cells could be reused for the removal of H2O2 from milk.
B S Kubal; S F D'Souza
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  59     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-05-11     Completed Date:  2005-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  61-4     Citation Subset:  IM    
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
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MeSH Terms
Catalase / chemistry*
Cell Membrane Permeability
Egg Proteins / chemistry
Enzymes, Immobilized / chemistry*
Food Technology / methods*
Glutaral / chemistry
Hydrogen Peroxide / isolation & purification
Milk / standards
Yeasts / cytology,  enzymology
Reg. No./Substance:
0/Egg Proteins; 0/Enzymes, Immobilized; 111-30-8/Glutaral; 7722-84-1/Hydrogen Peroxide; EC

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