| Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns. | |
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MedLine Citation:
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PMID: 20885470 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We developed a method for immobilization of biomolecules onto thiol functionalized surfaces according to UV diffraction patterns. UV light-assisted molecular immobilization proceeds through the formation of free, reactive thiol groups that can bind covalently to thiol reactive surfaces. We demonstrate that, by shaping the pattern of the UV light used to induce molecular immobilization, one can control the pattern of immobilized molecules onto the surface. Using a single-aperture spatial mask, combined with the Fourier transforming property of a focusing lens, we show that submicrometer (0.7 μm) resolved patterns of immobilized prostate-specific antigen biomolecules can be created. If a dual-aperture spatial mask is used, the results differ from the expected Fourier transform pattern of the mask. It appears as a superposition of two diffraction patterns produced by the two apertures, with a fine structured interference pattern superimposed. |
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Authors:
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Steffen Bjørn Petersen; Ane Kold di Gennaro; Maria Teresa Neves-Petersen; Esben Skovsen; Antonietta Parracino |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied optics Volume: 49 ISSN: 1539-4522 ISO Abbreviation: Appl Opt Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-01 Completed Date: 2011-01-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0247660 Medline TA: Appl Opt Country: United States |
Other Details:
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Languages: eng Pagination: 5344-50 Citation Subset: IM |
Affiliation:
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Nanobiotechnology Group, Department of Physics and Nanotechnology, Aalborg University, Skjernvej 4A, Aalborg, Denmark. sp@nano.aau.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Fourier Analysis Immobilized Proteins Lenses Photochemical Processes* Prostate-Specific Antigen / radiation effects* Sulfhydryl Compounds / pharmacology Surface Properties / drug effects Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/Immobilized Proteins; 0/Sulfhydryl Compounds; EC 3.4.21.77/Prostate-Specific Antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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