| Immobilization of Candida krusei cells producing phytase in alginate gel beads: an application of the preparation of myo-inositol phosphates. | |
| | |
MedLine Citation:
|
PMID: 12709834 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cells of Candida krusei capable of producing phytase were immobilized in Ca-alginate gel beads and used for the preparation of myo-inositol phosphates. The immobilization yield was increased about 5-fold after the beads were treated for 96 h at pH 4.0, 4 degrees C. The increased yield was retained, even after 1 month, when the cells were kept at this temperature and pH. No shift in the pH optima of phytase of the immobilized cells was observed, compared with that of free cells. However, the optimum temperature for the enzyme of the immobilized cells was 55 degrees C, which was 15 degrees C higher than that of free cells. The degradation characteristics of the phytate in immobilized cells packed in a glass column (i.d. 1.2 cm, length 20 cm) were investigated. The variation in the composition of the products results from a change in the flow rate of phytate solution (5 mM). At a flow rate of 1.30 ml/min, a mixture of myo-inositol-2-monophosphate, myo-inositol-1,2,5-triphosphate and myo-inositol-1,2,5,6-tetrakisphosphate was produced, in which the latter two were physiologically active. Also, it was found by NMR analysis that the enzyme of this strain produced only one isomer of each of the inositol phosphates, with the exception of myo-inositol pentakisphosphate. Therefore, the pure isomers were easily isolated using ion-exchange chromatography. |
| | |
Authors:
|
C S Quan; S D Fan; Y Ohta |
Related Documents
:
|
6440734 - The effect of solubilized bone matrix fractions from different mammalian species on gly... 10817344 - Determination of nadh-dependent glutamate synthase (gogat) in spodoptera frugiperda (sf... 1059054 - Separation of "sucrases" in supernatants of human dental plaque material and characteri... 8297334 - In vitro splicing of pre-messenger rna with extracts from 5-fluorouridine-treated cells. 1348694 - Annexin i and involucrin are cross-linked by particulate transglutaminase into the corn... 6440734 - The effect of solubilized bone matrix fractions from different mammalian species on gly... |
Publication Detail:
|
Type: Evaluation Studies; Journal Article Date: 2003-04-23 |
Journal Detail:
|
Title: Applied microbiology and biotechnology Volume: 62 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2003 Jul |
Date Detail:
|
Created Date: 2003-07-01 Completed Date: 2003-10-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
|
Languages: eng Pagination: 41-7 Citation Subset: IM |
Affiliation:
|
Graduate School of Biosphere Sciences, Hiroshima University, 1-4-4 Kagamiyama, 739-8528 Higashi-Hiroshima, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
6-Phytase
/
biosynthesis,
metabolism* Alginates / chemistry* Calcium / metabolism Candida / enzymology* Cells, Immobilized Culture Media Gels / chemistry Hydrogen-Ion Concentration Inositol Phosphates / biosynthesis, metabolism* Microspheres Temperature |
| Chemical | |
Reg. No./Substance:
|
0/Alginates; 0/Culture Media; 0/Gels; 0/Inositol Phosphates; 7440-70-2/Calcium; EC 3.1.3.26/6-Phytase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The role of gallium-67 scan in defining the extent of disease in an endemic deep mycosis, paracoccid...
Next Document: Differential methylation pattern of the X-linked lymphoproliferative (XLP) disease gene SH2D1A corre...