Document Detail


Immobilization of Candida krusei cells producing phytase in alginate gel beads: an application of the preparation of myo-inositol phosphates.
MedLine Citation:
PMID:  12709834     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells of Candida krusei capable of producing phytase were immobilized in Ca-alginate gel beads and used for the preparation of myo-inositol phosphates. The immobilization yield was increased about 5-fold after the beads were treated for 96 h at pH 4.0, 4 degrees C. The increased yield was retained, even after 1 month, when the cells were kept at this temperature and pH. No shift in the pH optima of phytase of the immobilized cells was observed, compared with that of free cells. However, the optimum temperature for the enzyme of the immobilized cells was 55 degrees C, which was 15 degrees C higher than that of free cells. The degradation characteristics of the phytate in immobilized cells packed in a glass column (i.d. 1.2 cm, length 20 cm) were investigated. The variation in the composition of the products results from a change in the flow rate of phytate solution (5 mM). At a flow rate of 1.30 ml/min, a mixture of myo-inositol-2-monophosphate, myo-inositol-1,2,5-triphosphate and myo-inositol-1,2,5,6-tetrakisphosphate was produced, in which the latter two were physiologically active. Also, it was found by NMR analysis that the enzyme of this strain produced only one isomer of each of the inositol phosphates, with the exception of myo-inositol pentakisphosphate. Therefore, the pure isomers were easily isolated using ion-exchange chromatography.
Authors:
C S Quan; S D Fan; Y Ohta
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2003-04-23
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  62     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-01     Completed Date:  2003-10-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  41-7     Citation Subset:  IM    
Affiliation:
Graduate School of Biosphere Sciences, Hiroshima University, 1-4-4 Kagamiyama, 739-8528 Higashi-Hiroshima, Japan.
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MeSH Terms
Descriptor/Qualifier:
6-Phytase / biosynthesis,  metabolism*
Alginates / chemistry*
Calcium / metabolism
Candida / enzymology*
Cells, Immobilized
Culture Media
Gels / chemistry
Hydrogen-Ion Concentration
Inositol Phosphates / biosynthesis,  metabolism*
Microspheres
Temperature
Chemical
Reg. No./Substance:
0/Alginates; 0/Culture Media; 0/Gels; 0/Inositol Phosphates; 7440-70-2/Calcium; EC 3.1.3.26/6-Phytase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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