| Imidazole groups on a linear, cyclodextrin-containing polycation produce enhanced gene delivery via multiple processes. | |
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MedLine Citation:
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PMID: 16891028 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The linear, cyclodextrin-containing polycation (CDP) is one of many non-viral gene delivery vectors that show improved transfection efficiency when modified to have pH-buffering capacity. The buffering activity is presumed to confer enhanced ability to escape the endocytic pathway. Here, the differences in delivery behavior between CDP and its pH-buffering, imidazole-containing variant (CDPim) are investigated in order to elucidate the mechanism(s) by which these related materials exhibit differences in gene delivery. In cell-free assays that include dye exclusion and heparan sulfate displacement, CDP appears to have weaker binding strength with nucleic acids than CDPim. Numerous analyses involving transfected cells, however, indicate that CDPim more readily releases nucleic acids in the intracellular setting. Together, these data suggest that differences in transfection efficiency between CDP and CDPim result from factors beyond buffering activity and endosomal escape. |
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Authors:
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Swaroop Mishra; Jeremy D Heidel; Paul Webster; Mark E Davis |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-27 |
Journal Detail:
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Title: Journal of controlled release : official journal of the Controlled Release Society Volume: 116 ISSN: 0168-3659 ISO Abbreviation: J Control Release Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-12-12 Completed Date: 2007-02-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8607908 Medline TA: J Control Release Country: Netherlands |
Other Details:
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Languages: eng Pagination: 179-91 Citation Subset: IM |
Affiliation:
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Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Buffers Chloroquine / chemistry Comet Assay / methods DNA / chemistry, genetics, metabolism* DNA Damage Flow Cytometry Hela Cells Heparitin Sulfate / chemistry Humans Hydrogen-Ion Concentration Imidazoles / chemistry* Intercalating Agents / chemistry Microscopy, Electron, Transmission Nucleic Acid Conformation Transfection / methods* Transport Vesicles / metabolism, ultrastructure beta-Cyclodextrins / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Imidazoles; 0/Intercalating Agents; 0/beta-Cyclodextrins; 54-05-7/Chloroquine; 9007-49-2/DNA; 9050-30-0/Heparitin Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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