Document Detail


Imbalance between the expression of NK1 and GABAB receptors in nociceptive spinal neurons during secondary hyperalgesia: a c-Fos study in the monoarthritic rat.
MedLine Citation:
PMID:  15857696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The neurochemical changes that operate in nociceptive spinal cord circuits during secondary hyperalgesia are largely unknown, in particular with respect to the balance between excitatory and inhibitory neurotransmission. In this study we evaluated the expression of NK1 and GABA(B) receptors in nociceptive spinal neurons in a model of secondary hyperalgesia consisting of noxious mechanical stimulation of the hindlimb skin close to a joint chronically inflamed by complete Freund's adjuvant. In spinal segments receiving input from that skin area, Fos-immunodetection was combined with immunocytochemistry for NK1 receptors, GABA(B) receptors or both receptors. In control and monoarthritic animals, neurons double-labeled for Fos and each receptor occurred mainly in laminae I and IV-V. In lamina I, the percentage of NK1 neurons expressing Fos was higher in monoarthritics while lower percentages of GABA(B) neurons expressed Fos. The percentage of Fos-positive cells expressing NK1 immunoreaction did not change in monoarthritics but that of Fos cells with GABA(B) immunoreaction was lower in these animals. In laminae IV-V, a large increase in Fos expression was detected in monoarthritic rats but the relative proportions of Fos-positive neurons expressing each receptor were similar in the two groups. Co-localization of NK1 and GABA(B) receptors occurred only in lamina I neurons in both experimental groups with no differences between control and monoarthritic animals in the percentages of Fos-positive neurons that expressed the receptors. Considering the participation of lamina I neurons bearing NK1 and GABA(B) receptors in several spinofugal systems, it is possible that the imbalance between excitatory and inhibitory actions exerted, respectively, by substance P and GABA may subserve secondary hyperalgesia by increasing ascending transmission of nociceptive input.
Authors:
A R Castro; M Pinto; D Lima; I Tavares
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience     Volume:  132     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  2005  
Date Detail:
Created Date:  2005-04-28     Completed Date:  2005-08-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  905-16     Citation Subset:  IM    
Affiliation:
Institute of Histology and Embryology, Faculdade de Medicina and IBMC, University of Porto, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arthritis / chemically induced,  complications
Disease Models, Animal
Freund's Adjuvant / toxicity
Hyperalgesia / etiology,  physiopathology*
Immunohistochemistry
Male
Neurons, Afferent / metabolism*
Nociceptors / metabolism
Proto-Oncogene Proteins c-fos / metabolism*
Rats
Rats, Wistar
Receptors, GABA-B / biosynthesis*
Receptors, Neurokinin-1 / biosynthesis*
Spinal Cord / metabolism*
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-fos; 0/Receptors, GABA-B; 0/Receptors, Neurokinin-1; 9007-81-2/Freund's Adjuvant

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