Document Detail


Imaging single mRNA molecules in yeast.
MedLine Citation:
PMID:  20946820     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Yeast cells in an isogenic population do not all display the same phenotypes. To study such variation within a population of cells, we need to perform measurements on each individual cell instead of measurements that average out the behavior of a cell over the entire population. Here, we provide the basic concepts and a step-by-step protocol for a recently developed technique enabling one such measurement: fluorescence in situ hybridization that renders single mRNA molecule visible in individual fixed cells.
Authors:
Hyun Youk; Arjun Raj; Alexander van Oudenaarden
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-03-01
Journal Detail:
Title:  Methods in enzymology     Volume:  470     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-10-15     Completed Date:  2011-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  429-46     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
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MeSH Terms
Descriptor/Qualifier:
Nucleic Acid Hybridization / methods*
RNA, Messenger / genetics*
Yeasts / genetics*
Grant Support
ID/Acronym/Agency:
R01-GM077183/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger

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