| Imaging single mRNA molecules in yeast. | |
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MedLine Citation:
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PMID: 20946820 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Yeast cells in an isogenic population do not all display the same phenotypes. To study such variation within a population of cells, we need to perform measurements on each individual cell instead of measurements that average out the behavior of a cell over the entire population. Here, we provide the basic concepts and a step-by-step protocol for a recently developed technique enabling one such measurement: fluorescence in situ hybridization that renders single mRNA molecule visible in individual fixed cells. |
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Authors:
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Hyun Youk; Arjun Raj; Alexander van Oudenaarden |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-03-01 |
Journal Detail:
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Title: Methods in enzymology Volume: 470 ISSN: 1557-7988 ISO Abbreviation: Meth. Enzymol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-10-15 Completed Date: 2011-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
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Languages: eng Pagination: 429-46 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Nucleic Acid Hybridization
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methods* RNA, Messenger / genetics* Yeasts / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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R01-GM077183/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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