| Imaging of single light-responsive clock cells reveals fluctuating free-running periods. | |
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MedLine Citation:
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PMID: 15738976 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Zebrafish tissues and cell lines contain circadian clocks that respond directly to light. Using fluorescence-activated cell sorting, we have isolated clonal cell lines that contain the reporter construct, zfperiod4-luciferase. Bioluminescent assays show that oscillations within cell populations are dampened in constant darkness. However, single-cell imaging reveals that individual cells continue to oscillate, but with widely distributed phases and marked stochastic fluctuations in free-running period. Because these cells are directly light responsive, we can easily follow phase shifts to single light pulses. Here we show that light acts to reset desynchronous cellular oscillations to a common phase, as well as stabilize the subsequent free-running period. |
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Authors:
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Amanda-Jayne F Carr; David Whitmore |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nature cell biology Volume: 7 ISSN: 1465-7392 ISO Abbreviation: Nat. Cell Biol. Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2005-03-01 Completed Date: 2005-03-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100890575 Medline TA: Nat Cell Biol Country: England |
Other Details:
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Languages: eng Pagination: 319-21 Citation Subset: IM |
Affiliation:
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University College London, Centre for Cell and Molecular Dynamics, Department of Anatomy and Developmental Biology, Rockefeller Building, 21 University Street, London WC1E 6DE, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Clocks Cell Separation Circadian Rhythm Fibroblasts / metabolism Flow Cytometry Light Luciferases / metabolism Microscopy, Fluorescence / methods* Nuclear Proteins / metabolism* Oscillometry Time Factors Zebrafish |
| Chemical | |
Reg. No./Substance:
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0/Nuclear Proteins; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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