Document Detail

Imaging scattering orientation with spatial frequency domain imaging.
MedLine Citation:
PMID:  22191918     Owner:  NLM     Status:  MEDLINE    
Optical imaging techniques based on multiple light scattering generally have poor sensitivity to the orientation and direction of microscopic light scattering structures. In order to address this limitation, we introduce a spatial frequency domain method for imaging contrast from oriented scattering structures by measuring the angular-dependence of structured light reflectance. The measurement is made by projecting sinusoidal patterns of light intensity on a sample, and measuring the degree to which the patterns are blurred as a function of the projection angle. We derive a spatial Fourier domain solution to an anisotropic diffusion model. This solution predicts the effects of bulk scattering orientation on the amplitude and phase of the projected patterns. We introduce a new contrast function based on a scattering orientation index (SOI) which is sensitive to the degree to which light scattering is directionally dependent. We validate the technique using tissue simulating phantoms, and ex vivo samples of muscle and brain. Our results show that SOI is independent of the overall amount of bulk light scattering and absorption, and that isotropic versus oriented scattering structures can be clearly distinguished. We determine the orientation of subsurface microscopic scattering structures located up to 600 μm beneath highly scattering (μ(') (s) = 1.5 mm(-1)) material.
Soren D Konecky; Tyler Rice; Anthony J Durkin; Bruce J Tromberg
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  16     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-23     Completed Date:  2012-05-04     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  126001     Citation Subset:  IM    
University of California, Irvine, Beckman Laser Institute and Medical Clinic, Laser Microbeam and Medical Program (LAMMP), 1002 Health Sciences Road, Irvine, California 92612, USA.
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MeSH Terms
Brain Chemistry
Diagnostic Imaging / methods*
Muscle, Skeletal / chemistry
Phantoms, Imaging
Rats, Sprague-Dawley
Reproducibility of Results
Scattering, Radiation*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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