| Imaging of nucleolar dynamics during the cell cycle of cancer cells in live mice. | |
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MedLine Citation:
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PMID: 17912040 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The synthesis and assembly of ribosomal subunits take place in the nucleolus. The nucleolus forms in the nucleus around the repeated ribosomal gene clusters and undergoes cyclic changes during the cell cycle. Although the nucleolus is easily visualized by light microscopy of cells in vitro, the nucleolus has not been imaged in cells in vivo. We report here development of a mouse model to visualize the nucleolus cycle of cancer cells in live mice. HT-1080 human fibrosarcoma cells were labeled in the nucleus with histone H2B-GFP and with retroviral RFP in the cytoplasm. The nucleolus was visualized by contrast to the fluorescence of GFP expressed in the nucleus. HT-1080 dual-color cells were seeded on the surface of a skin-flap of nude mice. The inside surface of the skin-flap was directly imaged with a laser scanning microscope 24 hours after seeding. The nucleoli of the cancer cells were clearly imaged in real-time. The appearance of the nucleoli changed dramatically during the cell cycle. During mitosis, the nucleolus disappeared. After mitosis, the nucleoli decreased in number and increased in size. The nucleolus appears to have a major role in cell cycle regulation. Nucleolar imaging could be used for more precise determination of cancer-cell position in the cell cycle in vivo. |
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Authors:
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Kensuke Yamauchi; Meng Yang; Katsuhiro Hayashi; Ping Jiang; Norio Yamamoto; Hiroyuki Tsuchiya; Katsuro Tomita; A R Moossa; Michael Bouvet; Robert M Hoffman |
Publication Detail:
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Type: Comparative Study; Journal Article Date: 2007-08-10 |
Journal Detail:
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Title: Cell cycle (Georgetown, Tex.) Volume: 6 ISSN: 1551-4005 ISO Abbreviation: Cell Cycle Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-12 Completed Date: 2007-12-31 Revised Date: 2008-09-23 |
Medline Journal Info:
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Nlm Unique ID: 101137841 Medline TA: Cell Cycle Country: United States |
Other Details:
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Languages: eng Pagination: 2706-8 Citation Subset: IM |
Affiliation:
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AntiCancer, Inc., San Diego, California 92111-3604, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle / physiology* Cell Line, Tumor Cell Nucleolus / chemistry*, physiology* Gene Expression Regulation, Neoplastic / physiology* Humans Mice Mice, Nude Microscopy, Confocal / methods |
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