Document Detail


Imaging the migrating border cell cluster in living Drosophila egg chambers.
MedLine Citation:
PMID:  17849456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell migration is a key process in animal development and central to the spread of cancer. Border cell migration in Drosophila egg chambers is an excellent general model for cell migration, but lacks techniques for studying this process in living cells. Here, we describe a simple and effective method of preparing egg chambers in halocarbon oil. The movement and behavior of the migrating border cells can reproducibly be followed in up to 25 egg chambers simultaneously by time-lapse microscopy using a variety of green fluorescent protein markers on a widefield microscope over a period of 4 hr. Our studies reveal a remarkably linear migration route of the border cell cluster and highly dynamic activity within their cluster. Migrating cells rapidly alter their relative positions and generate transient protrusions. These activities are likely to play key roles in the mechanism of migration and cannot readily be analyzed using fixed samples.
Authors:
Hille Tekotte; David Tollervey; Ilan Davis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  236     ISSN:  1058-8388     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-01     Completed Date:  2008-01-09     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2818-24     Citation Subset:  IM    
Copyright Information:
2007 Wiley-Liss, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement*
Drosophila / cytology*,  physiology
Female
Green Fluorescent Proteins
Microscopy, Interference / methods*
Oogenesis
Ovary / cytology
Grant Support
ID/Acronym/Agency:
067413//Wellcome Trust; 081858//Wellcome Trust; G0001292//Medical Research Council
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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