Document Detail

Imaging of mesoscopic-scale organisms using selective-plane optoacoustic tomography.
MedLine Citation:
PMID:  19369709     Owner:  NLM     Status:  MEDLINE    
Mesoscopic-scale living organisms (i.e. 1 mm to 1 cm sized) remain largely inaccessible by current optical imaging methods due to intensive light scattering in tissues. Therefore, imaging of many important model organisms, such as insects, fishes, worms and similarly sized biological specimens, is currently limited to embryonic or other transparent stages of development. This makes it difficult to relate embryonic cellular and molecular mechanisms to consequences in organ function and animal behavior in more advanced stages and adults. Herein, we have developed a selective-plane illumination optoacoustic tomography technique for in vivo imaging of optically diffusive organisms and tissues. The method is capable of whole-body imaging at depths from the sub-millimeter up to centimeter range with a scalable spatial resolution in the order of magnitude of a few tenths of microns. In contrast to pure optical methods, the spatial resolution here is not determined nor limited by light diffusion; therefore, such performance cannot be achieved by any other optical imaging technology developed so far. The utility of the method is demonstrated on several whole-body models and small-animal extremities.
Daniel Razansky; Claudio Vinegoni; Vasilis Ntziachristos
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Publication Detail:
Type:  Journal Article     Date:  2009-04-15
Journal Detail:
Title:  Physics in medicine and biology     Volume:  54     ISSN:  0031-9155     ISO Abbreviation:  Phys Med Biol     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-22     Completed Date:  2009-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401220     Medline TA:  Phys Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2769-77     Citation Subset:  IM    
Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany.
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MeSH Terms
Drosophila / anatomy & histology,  growth & development
Oligochaeta / anatomy & histology
Tomography / methods*

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