| Imaging of mRNA in live cells using nucleic acid-templated reduction of azidorhodamine probes. | |
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MedLine Citation:
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PMID: 19378999 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleic acid-templated reactions leading to a fluorescent product represent an attractive strategy for the detection and imaging of cellular nucleic acids. Herein we report the use of a Staudinger reaction to promote the reduction of profluorescent azidorhodamine. The use of two cell-permeable GPNA probes, one labeled with the profluorescent azidorhodamine and the other with trialkylphosphine, enabled the detection of the mRNA encoding O-6-methylguanine-DNA methyltransferase in intact cells. |
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Authors:
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Zbigniew Pianowski; Katarzyna Gorska; Laurence Oswald; Christoph A Merten; Nicolas Winssinger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 131 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-05-06 Completed Date: 2009-07-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 6492-7 Citation Subset: IM |
Affiliation:
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Institut de Science et Ingenierie Supramoleculaires (ISIS-UMR 7006), Universite de Strasbourg-CNRS, 8 allee Gaspard Monge, 67000 Strasbourg, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Cells / chemistry, cytology* DNA Probes Diagnostic Imaging / methods Fluorescent Dyes Humans Molecular Probe Techniques* O(6)-Methylguanine-DNA Methyltransferase / analysis RNA, Messenger / analysis* Rhodamines / diagnostic use* |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 0/Fluorescent Dyes; 0/RNA, Messenger; 0/Rhodamines; EC 2.1.1.63/O(6)-Methylguanine-DNA Methyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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