Document Detail


Imaging of lysophosphatidylcholine in human coronary plaques by color fluorescence angioscopy.
MedLine Citation:
PMID:  20379048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lysophosphatidylcholine (LPC) is a proinflammatory and proatherogenic substance, and it plays an important role in the initiation, progression, and destabilization of atherosclerotic plaques. If LPC in the vascular wall is visualized in vivo, the mechanisms of atherosclerosis and the effects of medical and interventional therapies on atherosclerosis can be objectively evaluated. Therefore, this study was carried out to visualize LPC in human coronary plaques using a color fluorescence angioscopy (CFA) system. (1) The fluorescence characteristics of LPC were investigated by color fluorescence microscopy (CFM) using Trypan blue dye (TB) as an indicator. For fluorescence imaging, a combination of a band-pass filter (345 nm) and a band-absorption filter of 420 nm (A imaging), or a combination of a band-pass filter (470 nm) and a band-absorption filter of 520 nm (B imaging) was employed. (2) The fluorescence of LPC in the excised human coronary plaques was investigated by CFA and CFM scanning using the same filters as those in CFM. In the presence of TB, LPC exhibited a red fluorescence in both A and B imaging. This red fluorescence color in both A and B imaging was not observed for the other known major substances that constitute the atherosclerotic plaques. This red fluorescence color in both A and B imaging was detected by CFA in both white and yellow plaques that were classified by conventional angioscopy. This fluorescence color was found to be distributed in a web-like or diffuse configuration by CFM scanning. LPC in the human coronary plaques was successfully visualized by CFA using TB as an indicator.
Authors:
Yasumi Uchida; Yasuto Uchida; Seiji Kawai; Ryohei Kanamaru; Noriaki Kameda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International heart journal     Volume:  51     ISSN:  1349-2365     ISO Abbreviation:  Int Heart J     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-09     Completed Date:  2010-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101244240     Medline TA:  Int Heart J     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  129-33     Citation Subset:  IM    
Affiliation:
Japan Foundation for Cardiovascular Research, Funabashi, Chiba, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aged
Angioscopes*
Angioscopy / methods*
Coloring Agents / diagnostic use
Coronary Artery Disease / metabolism*,  pathology*
Female
Humans
Lysophosphatidylcholines / metabolism*
Male
Microscopy, Fluorescence*
Middle Aged
Tissue Culture Techniques
Trypan Blue / diagnostic use
Chemical
Reg. No./Substance:
0/Coloring Agents; 0/Lysophosphatidylcholines; 72-57-1/Trypan Blue

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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