Document Detail


Imaging of long-distance alpha-aminoisobutyric acid translocation dynamics during resource capture by Serpula lacrymans.
MedLine Citation:
PMID:  18344339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
alpha-Aminoisobutyric acid (AIB) is a nonmetabolized amino acid analogue of alanine, which at low (muM) concentrations acts as a tracer for amino acid movements. At high concentrations (mM), it competitively inhibits membrane transport and metabolism of protein amino acids and acts as a systemic translocated inhibitor of mycelial extension in fungi. AIB can control mycelial spread of the basidiomycete Serpula lacrymans, the cause of brown rot of wood in buildings. However, it is not known how effectively the inhibitor is distributed throughout the mycelium. Realistically heterogeneous microcosms, in which the fungus grew across nutritionally inert sand to colonize discrete wood resources, were used to investigate patterns of inhibition and translocation following local application of AIB. At a 0.1 M concentration, locally applied AIB caused immediate arrest of extension throughout the whole mycelium, maintained for a 6-week experimental period. The dynamics of translocation of subtoxic amounts of [1-(14)C]AIB ([(14)C]AIB) were mapped by photon-counting scintillation imaging in conjunction with destructive harvest to establish the velocity, direction, and rate of translocation and the extent of [(14)C]AIB reallocation accompanying the invasion of fresh wood. Locally applied [(14)C]AIB was distributed throughout complex mycelial networks within 2 h of application, becoming localized in growing margins by 12 h. Encounter with a fresh wood resource triggered a widespread response, causing withdrawal of [(14)C]AIB from throughout the network, accompanied by accumulation in the newly colonized wood and associated mycelium. The results are discussed in the context of nutrient dynamics in wood decomposer fungi and the mechanism of the amino acid reallocation response.
Authors:
Monika Tlalka; Mark Fricker; Sarah Watkinson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-14
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  74     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-29     Completed Date:  2008-05-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2700-8     Citation Subset:  IM    
Affiliation:
Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, United Kingdom. sarah.watkinson@plants.ox.ac.uk.
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MeSH Terms
Descriptor/Qualifier:
Aminoisobutyric Acids / metabolism*,  pharmacology*
Antifungal Agents / metabolism*,  pharmacology*
Basidiomycota / chemistry,  drug effects*,  growth & development,  metabolism*
Carbon Radioisotopes / metabolism
Isotope Labeling
Models, Biological
Mycelium / chemistry,  drug effects,  growth & development
Grant Support
ID/Acronym/Agency:
43/P19284//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Aminoisobutyric Acids; 0/Antifungal Agents; 0/Carbon Radioisotopes
Comments/Corrections

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